Učinak pentadekapeptida BPC 157 na posljedice kauterizacije episkleralnih vena štakora [The effect of pentadecapeptide BPC 157 on consequences of episcleral vein cauterization in rats]

Kralj, Tamara (2019) Učinak pentadekapeptida BPC 157 na posljedice kauterizacije episkleralnih vena štakora [The effect of pentadecapeptide BPC 157 on consequences of episcleral vein cauterization in rats]. PhD thesis, Sveučilište u Zagrebu.

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Abstract

In female Wistar rats, 220 – 250 g, two dorsal and one temporal episcleral vein were isolated and cauterized. Medication was BPC 157 (10 μg/kg or 10 ng/kg) intraperitoneally (5 ml/kg) or locally (2x2 drops per day), either immediately before surgery and then once time daily; or at 24 h after surgery and then once time daily. Controls simultaneously received an equal volume of saline. Intraocular pressure, pupillar function were measured 24 hours, 4 and 6 weeks after procedure. Non-invasive IOP measurements were taken by aplanation tonometer “Tonopen XL”. Pupillar function was photographed by USB “Veho Discovery VMS-004 Deluxe” microcamera. Photographs were taken before and after medication application and analyzed with special camera software for measurement of pupillar diameter (r) in mm, range (C) in mm and surface (S) in mm2. Camera was previously calibrated using graph paper. Vascularization of the eye fundus and presentation of optic disc were analyzed with microcamera and “VOLK Digital Widefield lupe” 90D. Pathohistological analysis was performed after sacrifice. Episcleral vein cauterization caused high intraocular pressure, changes in pupillar function, retinal ischemia and optic nerve disk atrophy. BPC 157 applied locally or intraperitoneally normalized intraocular pressure, pupillar function, antagonized retinal ischemia and optic nerve atrophy.

Abstract in Croatian

Ženke Wistar štakora, 220 – 250 g, raspoređene su na tretirane i kontrolne skupine. Model glaukoma u štakora izazvan je kauterizacijom episkleralnih vena: dvije dorzalne vene locirane u blizini m. rectus superiora i jednu inferiornu episkleralnu venu u blizini m. rectus lateralisa. Neposredno prije zahvata prva skupina tretiranih životinja, te nakon toga svakodnevno, primila je BPC 157 (10 μg/kg ili 10 ng/kg), intraperitonealno ili lokalno u obliku kapi 2x2 dnevno. Druga skupina životinja 24 h nakon zahvata primila je pentadekapeptid BPC 157 (10 μg/kg), te nakon toga svakodnevno intraperitonealno ili lokalno u obliku kapi 2x2 dnevno. Kontrolne životinje su istovremeno intraperitonealno ili lokalno primale jednaki volumen 0,9% NaCl. U intervalima od 24 sata, 4 tjedna i 6 tjedana analizirani su intraokularni tlak, funkcija zjeničnog sfinktera, krvne žile fundusa i disk vidnog živca te histološka prezentacija retine. Neinvazivno mjerenje očnog tlaka izvršeno je aplanacijskim tonometrom „Tonopen XL“. Snimanje zjenica mikrokamerom učinjeno je USB „Veho Discovery VMS-004 Deluxe“ te su polumjer (r) u mm, opseg (C) u mm i površina zjenica (S) u mm2 izmjereni posebnim softverom kupljenim s kamerom. Očna pozadina analizirana je mikrokamerom i „VOLK Digital Widefield“ 90D lupom te patohistološkom analizom bulbusa na standardnim rezovima, uklopljenog u parafin i bojenog hemalaun eozinom 24 sata, 4 tjedna i 6 tjedana nakon zahvata. Kauterizacija episkleralnih vena uzrokovala je povišenje očnog tlaka, proširenje zjenice, ishemiju očne pozadine i atrofiju diska vidnog živca. Intraperitonealna ili lokalna primjena BPC 157 dovela je do normalizacije očnog tlaka, oporavka zjeničnih funkcija, antagonizacije mrežnične ishemije te je spriječila atrofiju vidnog živca.

Item Type: Thesis (PhD)
Mentors:
Mentor
Sikirić, Predrag
Departments: Izvan medicinskog fakulteta
Depositing User: Anja Majstorović
University: Sveučilište u Zagrebu
Institution: Medicinski fakultet
Number of Pages: 127
Status: Unpublished
Creators:
CreatorsEmail
Kralj, TamaraUNSPECIFIED
Date: 3 April 2019
Date Deposited: 03 Jul 2019 08:52
Last Modified: 02 Sep 2019 07:40
Subjects: /
Related URLs:
    URI: http://medlib.mef.hr/id/eprint/3332

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