Morphometric and DNA image analysis of bronchoalveolar lavage fluid macrophages nuclei in interstitial lung diseases with lymphocytic alveolitis [Morfometrijska i DNA image analiza jezgara makrofaga u bronhoalveolarnom lavatu bolesnika sa bolestima plućnog intersticija i limfocitnim alveolitisom]

Smojver-Ježek, Silvana and Peroš-Golubičić, Tatjana and Tekavec-Trkanjec, Jasna and Alilović, Marija and Vrabec-Branica, Božica and Juroš, Zrinka and Mažuranić, Ivica (2010) Morphometric and DNA image analysis of bronchoalveolar lavage fluid macrophages nuclei in interstitial lung diseases with lymphocytic alveolitis [Morfometrijska i DNA image analiza jezgara makrofaga u bronhoalveolarnom lavatu bolesnika sa bolestima plućnog intersticija i limfocitnim alveolitisom]. Collegium Antropologicum, 34 (1). pp. 123-129. ISSN 0350-6134

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Abstract

Lymphocytic alveolitis is a characteristic of diverse interstitial lung diseases (ILD-s), but macrophages are often more numerous cell population in bronchoalveolar lavage fluid (BALF). Aim of this study is to analyze morphometric characteristics of macrophages nuclei in BALF in patients with ILD-s and to detect possible differences allowing distinguishing sarcoidosis from other lymphocytic alveolitis ILD-s. Thirty-one patient with interstitial lung disease who had lymphocytic alveolitis in BALF cell count (17 sarcoidosis and 14 other ILD-s) and nine controls were included in the study. The following patients data were numbered: age, lymphocyte percentage and CD4/CD8 ratio in BALF. Investigated morphometric parameters of macrophages nuclei were: area, outline, maximal radius, minimal radius, length, breadth, form factor (FF), elongation factor (EF) and DNA image cytometry ploidy status determined with Van Velthoven method. Predicted classifications in classification matrix (forward step-wise method in multivariate discriminant function analysis) based on macrophages nuclei length mean, minimum and maximum, breadth SD, FF mean and lymphocyte % were 100% (9/9) correct for control group, 88.235% (15/17) correct for sarcoidosis, and 92.857% (13/14) correct for other lymphocytic alveolitis ILD group. In total, 92.5% (37/40) of the examinees were correctly classified in particular group upon the observed variables.

Abstract in Croatian

Makrofagi su u većine bolesti plućnog intersticija, kao i u limfocitnom alveolitisu, najbrojnija stanična populacija u bronhoalveolarnom lavatu. Cilj rada bio je uz morfometrijsku analizu jezgara makrofaga u bronhoalveolarnom lavatu bolesnika sa bolestima plućnog intersticija procijeniti mogućnosti razlikovanja sarkoidoze od drugih bolesti intersticija koje se prezentiraju limfocitnim alveolitisom. U ispitivanje je uključen trideset i jedan bolesnik sa bolestima plućnog intersticija i limfocitnim alveolitisom u bronhoalveolarnim lavatu (17 bolesnika sa sarkoidozom i 14 bolesnika sa drugim bolestima) i 9 kontrolnih ispitanika. Bilježeni su dob bolesnika, postotak limfocita i omjer CD4 i CD8 T limfocita u bronhoalveolarnom lavatu. Analizirani su morfometrijski parametari jezgara makrofaga: površina, opseg, najveći polumjer, najmanji polumjer, duljina, širina, faktor oblika (FF), faktor izduženosti (EF), te DNA indeks određen metodom po Van Velthovenu. Multivariatnom diskriminativnom analizom uz korištenje stupnjevitog eliminiranja identificirane su varijable: prosjek, minimalne i maksimalne vijednosti duljine, SD širine, prosjek faktora oblika (FF) i prosjek postotka limfocita. Ove varijble omogućuju razlikovanje pojedinih skupina: potpuno razlikovanje (9/9) kontrolne skupine, 88,235% (15/17) razlikovanje skupine bolesnika sa sarkoidozom i 92,857% (13/14) skupine sa drugim bolestima plućnog intersticija i limfocitnim alveolitisom. Identificirane varijable omogućuju točno klasificiranje 92,5% (37/40) ispitanika.

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