Foetal Leydig cells and the neuroendocrine system

Šklebar, Duška and Šemanjski, Kristina and Kos, Marina and Šklebar, Ivan and Ježek, Davor (2008) Foetal Leydig cells and the neuroendocrine system. Collegium Antropologicum, 32 (S. 1). pp. 149-153. ISSN 0350-6134

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Abstract

It has been shown that adult human Leydig cells express a number of neuroendocrine markers, and, therefore, could be considered as a part of the neuroendocrine system in the adult. A limited number of studies have dealt with the dynamics of development of human foetal Leydig cells, whereas studies on their neuroendocrine nature are still extremely rare. Therefore, the aim of our study was to investigate the development of human foetal Leydig cells in different weeks of gestation (wg) and to check if these cells express certain markers characteristic of the diffuse neuroendocrine system (DNS). Qualitative, quantitative histological studies and immunohistochemical analyses of human foetal testicular tissue have been performed. According to our data, Leydig cells formed a dynamic population of cells within the interstitum of testes in the period between 15 and 36 wg. The total number of Leydig cells of human foetal testes changed through different stages of gestation by means of 'pulsatile' dynamics (most likely, by following the variable level of gonadotropins). At early stages of development (15-17 wg) immunohistochemical reactions for the expression of neuron specific enolase (NSE) were positive within sex cords and between them, in the interstitum. Pro-spermatogonia in the sex cords were positive, as well as elongated spindle-shaped cells of the interstitum (very likely, precursors of Leydig cells). During the later stages of development (28-36 wg), excluding the pro-spermatogonia, the interstitial Leydig cells were also positive. The results of the immunohistochemical analyses (the expression of NSE) confirmed the hypothesis that human foetal Leydig cells were of neuroendocrine nature.

Abstract in Croatian

Ljudske Leydigove stanice u odraslom sjemeniku posjeduju izražaj neuroendokrinih markera. Stoga se smatraju dijelom neuroendokrinog sustava odrasle individue. Manji broj istraživanja bavio se razvojem ljudskih fetalnih Leydigovih stanica, dok su studije o neuroendokrinim obilježjima tih stanica izrazito rijetke. Zato je cilj našeg istraživanja bio istražiti razvoj ljudskih fetalnih Leydigovih stanica tijekom različitih tjedana gestacije i provjeriti da li ove stanice izražavaju određene molekule/markere karakteristične za difuzni neuroendokrini sustav (DNS). Načinjena je kvalitativna, kvantitativna i imunohistokemijska analiza tkiva ljudskog fetalnog sjemenika. Prema našim podacima, fetalne Leydigove stanice u razdoblju od 15. do 36. tjedna gestacije čine dinamičnu skupinu stanica u intersticiju sjemenika. Ukupan broj Leydigovih stanica ljudskih sjemenika povećavao se »pulzatilno« (najvjerovatnije slijedeći promjenjivu razinu gonadotropina). U ranim stadijima razvoja (od 15. do 17. tjedna gestacije) imunohistokemijski izražaj neuron specifične enolaze (NSE) bio je pozitivan u stanicama spolnih tračaka i između njih, u intersticiju. Unutar spolnih tračaka pozitivne su bile prospermatogonije, dok su u intersticiju pozitivne bile izduljene stanice (najvjerovatnije preteče Leydigovih stanica). U kasnijim fazama razvoja (od 28. do 36. tjedna gestacije), osim prospermatogonija, pozitivne su bile i intersticijske Leydigove stanice. Rezultati imunohistokemijske analize (izražaj NSE) potvrdili su hipotezu o neuroendokrinoj prirodi ljudskih fetalnih Leydigovih stanica.

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