Božinović, Ksenija and Sabol, Ivan and Dediol, Emil and Milutin Gašperov, Nina and Manojlović, Spomenka and Vojtechova, Zuzana and Tachezy, Ruth and Grce, Magdalena (2019) Genome-wide miRNA profiling reinforces the importance of miR-9 in human papillomavirus associated oral and oropharyngeal head and neck cancer. Scientific Reports, 9 (1). ISSN 2045-2322
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Abstract
Head and neck cancer is the sixth most common malignancy worldwide, predominantly developing from squamous cell epithelia (HNSCC). The main HNSCC risk factors are tobacco, excessive alcohol use, and the presence of human papillomavirus (HPV). HPV positive (+) cancers are etiologically different from other HNSCC and often show better prognosis. The current knowledge regarding HNSCC miRNA profiles is still incomplete especially in the context of HPV+ cancer. Thus, we analyzed 61 freshly collected primary oral (OSCC) and oropharyngeal (OPSCC) SCC samples. HPV DNA and RNA was found in 21% cases. The Illumina whole-genome small-RNA profiling by next-generation sequencing was done on 22 samples and revealed 7 specific miRNAs to HPV+ OSCC, 77 to HPV+ OPSCC, and additional 3 shared with both; 51 miRNAs were specific to HPV− OPSCC, 62 to HPV− OSCC, and 31 shared with both. The results for 9 miRNAs (miR-9, -21, -29a, -100, -106b, -143 and -145) were assessed by reverse transcription-quantitative polymerase chain reaction on the whole study population. The data was additionally confirmed by reanalyzing publicly available miRNA sequencing Cancer Genome Atlas consortium (TCGA) HNSCC data. Cell signaling pathway analysis revealed differences between HPV+ and HPV− HNSCC. Our findings compared with literature data revealed extensive heterogeneity of miRNA deregulation with only several miRNAs consistently affected, and miR-9 being the most likely HPV related miRNA.
| Item Type: | Article | ||||||||||||||||||
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| Additional Information: | Open Access. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. © The Author(s) 2019 | ||||||||||||||||||
| Departments: | Katedra za maksilofacijalnu kirurgiju sa stomatologijom Katedra za patologiju |
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| Depositing User: | Kristina Berketa | ||||||||||||||||||
| Status: | Published | ||||||||||||||||||
| Creators: |
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| Date: | 19 February 2019 | ||||||||||||||||||
| Date Deposited: | 27 Nov 2019 12:06 | ||||||||||||||||||
| Last Modified: | 27 Nov 2019 12:06 | ||||||||||||||||||
| Subjects: | / | ||||||||||||||||||
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| URI: | http://medlib.mef.hr/id/eprint/3511 |
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