Koppes, Sjors A. and Ljubojević Hadžavdić, Suzana and Jakasa, Ivone and Franceschi, Nika and Riethmüller, Christoph and Jurakić Tončic, Ružica and Marinović, Branka and Raj, Nidhin and Rawlings, Anthony V. and Voegeli, Rainer and Lane, Majella E. and Haftek, Marek and Frings-Dresen, Monique H.W. and Rustemeyer, Thomas and Kezic, Sanja (2017) Effect of allergens and irritants on levels of natural moisturizing factor and corneocyte morphology. Contact Dermatitis, 76 (5). pp. 287-295. ISSN 0105-1873
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Abstract
BACKGROUND: The irritant sodium lauryl sulfate (SLS) is known to cause a decrease in the stratum corneum level of natural moisturizing factor (NMF), which in itself is associated with changes in corneocyte surface topography. ----- OBJECTIVE: To explore this phenomenon in allergic contact dermatitis. ----- METHODS: Patch testing was performed on patients with previously positive patch test reactions to potassium dichromate (Cr), nickel sulfate (Ni), methylchloroisothiazolinone (MCI)/methylisothiazolinone (MI), or p-phenylenediamine. Moreover, a control (pet.) patch and an irritant (SLS) patch were applied. After 3 days, the stratum corneum from tested sites was collected, and NMF levels and corneocyte morphology, expressed as the amount of circular nanosize objects, quantified according to the Dermal Texture Index (DTI), were determined. ----- RESULTS: Among allergens, only MCI/MI reduced NMF levels significantly, as did SLS. Furthermore, only MCI/MI caused remarkable changes at the microscopic level; the corneocytes were hexagonal-shaped with pronounced cell borders and a smoother surface. The DTI was increased after SLS exposure but not after allergen exposure. ----- CONCLUSIONS: MCI/MI significantly decreased NMF levels, similarly to SLS. The altered corneocyte morphology suggests that skin barrier damage plays a role in the pathogenesis of MCI/MI contact allergy. The DTI seems to differentiate reactions to SLS from those to the allergens tested, as SLS was the only agent that caused a DTI increase.
| Item Type: | Article | ||||||||||||||||||||||||||||||||
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| Additional Information: | © 2017 The Authors. Contact Dermatitis published by John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. | ||||||||||||||||||||||||||||||||
| MeSH: | Allergens/adverse effects ; Allergens/immunology ; Dermatitis, Allergic Contact/diagnosis ; Dermatitis, Allergic Contact/etiology ; Epidermis/drug effects ; Humans ; Irritants/adverse effects ; Irritants/pharmacology ; Patch Tests ; Skin Physiological Phenomena ; Sodium Dodecyl Sulfate/adverse effects ; Sodium Dodecyl Sulfate/pharmacology | ||||||||||||||||||||||||||||||||
| Departments: | Katedra za dermatovenerologiju | ||||||||||||||||||||||||||||||||
| Depositing User: | Kristina Berketa | ||||||||||||||||||||||||||||||||
| Status: | Published | ||||||||||||||||||||||||||||||||
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| Date: | May 2017 | ||||||||||||||||||||||||||||||||
| Date Deposited: | 17 Oct 2019 11:14 | ||||||||||||||||||||||||||||||||
| Last Modified: | 20 Aug 2020 06:59 | ||||||||||||||||||||||||||||||||
| Subjects: | / | ||||||||||||||||||||||||||||||||
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| URI: | http://medlib.mef.hr/id/eprint/3460 |
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