Nationwide survey of Klebsiella pneumoniae strains producing CTX-M extended-spectrum b-lactamases in Croatia [Nacionalno istraživanje sojeva bakterije klebsiella pneumoniae koji proizvode CTX-M prošireni-spektar b-laktamaza u Hrvatskoj]

Fiolić, Zlatko and Bošnjak, Zrinka and Bedenić, Branka and Budimir, Ana and Mareković, Ivana and Ćetković, Helena and Kalenić, Smilja (2015) Nationwide survey of Klebsiella pneumoniae strains producing CTX-M extended-spectrum b-lactamases in Croatia [Nacionalno istraživanje sojeva bakterije klebsiella pneumoniae koji proizvode CTX-M prošireni-spektar b-laktamaza u Hrvatskoj]. Collegium Antropologicum, 39 (4). pp. 947-951. ISSN 0350-6134

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Abstract

Extended-spectrum β-lactamases (ESBL) producing bacteria have been increasingly reported in both hospital and community patients. Production of ESBLs is the major mechanism of resistance to oxymino-cephalosporins and aztreonam in Gram-negative bacteria. Recently a new family of ESBLs with predominant activity against cefotaxime (CTX-M β-lactamases) has been reported. Over 80 CTX-M enzymes have been described so far, which can be grouped into five main subgroups according to amino acid sequence identity (CTX-M-1, CTX-M-2, CTX-M-8, CTX-M-9 and CTX-M-25). In some countries, CTX-M β-lactamases are the most prevalent types of ESBLs, for instance in Russia, Greece, Spain, Switzerland, Japan, Taiwan, China and Argentina. These enzymes have been identified in countries near Croatia such is Italy, Hungary and Austria. The aim of this study was to determine the prevalence and the types of CTX-M β lactamases produced by Klebsiella pneumoniae clinical isolates collected from October 2006 to January 2007 from both community- and hospital-based isolates were included (Figure 1.). 128 ESBL isolates were subjected to further analysis: screening with double disc diffusion test and confirmed by ESBL E test.

Abstract in Croatian

Prošireni spektar beta-laktamaza (ESBL) kojr proizvode bakterije se povišeno pojavljuje kod bolničkih pacijenta I pacijenata u zajednici. Proizvodnja ESBL-a je glavni mehanizam otpornosti na oxymino-cefalosporine i aztreoname kod gram-negativnih bakterija1,2. Nedavno je zabilježena nova obitelj ESBL-a s dominantnim djelovanjem protiv cefotaksima (CTX-M beta-laktamaze). Više od 80 CTX-M enzima je do sada opisano, koji se mogu grupirati u pet glavnih podskupina prema istovjetnosti aminokiselinskog slijeda (CTX-M-1, CTX-M-2, CTX-M-8, CTX-M-9 i CTX-M-25)3. U nekim zemljama, CTX-M beta-laktamaze su najzastupljenije vrste ESBL-a, primjerice u Rusiji4, Grčkoj5, Španjolskoj6, Švicarskoj7 , Japanu8, Tajvanu9 , Kini10 i Argentini11. Ovi enzimi su identificirani u zemljama u blizini Hrvatske poput Italije12, Madžarske13 i Austrije14. Cilj ovog istraživanja bio je utvrditi učestalost i vrste CTX-M beta laktamaza koje proizvode klinički izolati bakterjie Klebsiella pneumoniae prikupljeni od listopada 2006. do siječnja 2007. godine od oba u zajednici i bolnica-based izolata su bili uključeni (slika 1.). 128 ESBL izolati bili podvrgnuti daljnjoj analizi: screening s dvostrukim disk difuzije testa i potvrđuje ESBL E test.

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