Spolne razlike u osteotropnim elementima u ljudskoj kosi i krvi [Gender dependent differences in osteotrophic elements in human hair and blood]

Prejac, Juraj (2018) Spolne razlike u osteotropnim elementima u ljudskoj kosi i krvi [Gender dependent differences in osteotrophic elements in human hair and blood]. PhD thesis, Sveučilište u Zagrebu.

PDF Download (9MB)

Abstract

Metabolic changes related to age and gender are becoming a public health and clinical problem. Reliable bioindicators for individual assessment of bioelement metabolic status are needed. Blood and hair are two easily accessible tissues suitable for metabolic status assesment. The study included 192 subjects (83 men, 109 women). The samples were analyzed with the ICP-MS highlighting the osteotrophic elements (B, Ba, Ca, Mg, P, Si, Sr, and Zn) as the primary research objectives. The accumulation of bioelements (Ca>Zn>P>Mg>Si>Sr>B>Ba) appeared significantly higher in the hair than the blood. This fact makes hair a more suitable biological matrix for chemical analysis of the studied elements than whole blood. For the first time the normal concentration ranges of bioelements were defined by analyzing the frequency distribution properties in the hair and whole blood. At the same time, the concentration limits for bioelement deficiency and excess were defined. Especially significant is the finding of higher concentrations of elements belonging to the 2A group of the Periodic table of elements (Ca>Mg>Sr>Ba) in women's hair compared to men's. The ratio of osteotrophic bioelements within the same gender remained unchanged. Evidently, the metabolic control of mineralization of the organic biological matrix differs substantially between the genders. No such differences were observed in the whole blood.

Abstract in Croatian

Promjene u metabolizmu vezane uz dob i spol, javno su zdravstveni i klinički problem. Potrebni su pouzdani bioindikatori za individualnu procjenu metaboličkog statusa bioelemenata, a krv i kosa lako su dostupna tkiva prikladna za određivanje metaboličkog statusa bioelementnih profila osteotropnih elemenata. U istraživanje multi-bioelementnog profila osteotropnih elemenata bila su uključena 192 ispitanika (83 muškarca, 109 žena). Uzorci su analizirani metodom ICP-MS i izdvojeni su oni bioelementi sa ulogom u metabolizmu kosti: B, Ba, Ca, Mg, P, Si, Sr i Zn. Analiza nakupljanja bioelemenata u kosi i krvi (Ca>Zn>P>Mg>Si>Sr>B>Ba) pokazala je da je njihova koncentracija značajno viša u kosi nego u krvi. To kosu čini pogodnijom organsko-biološkom matricom od krvi za kemijsku analizu bioelemenata. Analizirajući frekvenciju distribucije metodom derivata medijana, po prvi puta su jasno definirani adekvatni rasponi koncentracije bioelemenata u kosi i punoj krvi. Istodobno, precizirane su i granično niske koncentracije ispitanih bioelemenata ispod kojih govorimo o deficitu, kao i povišenim vrijednostima kada govorimo o prekomjernoj izloženosti nekom elementu. Osobito značajnim smatramo nalaz povišenih koncentracija elemenata skupine 2A Periodičkog sustava (Ca>Mg>Sr>Ba) u kosi žena naspram kose muškaraca. Pri tomu je njihov omjer unutar istoga spola bio konstantan. Očigledno je kako je metabolička kontrola mineralizacije organske biološke matrice kose bitno različita između muškaraca i žena. Analizom elemenata u krvi, nismo opazili takve razlike.

Actions (login required)

View Item

Downloads