Usporedba izraženosti koneksina 43 u tkivu karcinoma debelog crijeva u bolesnika sa sinkronim jetrenim presadnicama i bez njih [Comparison of connexin 43 expression in colorectal carcinomas in patients with and without synchronous liver metastases]

Radić, Jasna (2016) Usporedba izraženosti koneksina 43 u tkivu karcinoma debelog crijeva u bolesnika sa sinkronim jetrenim presadnicama i bez njih [Comparison of connexin 43 expression in colorectal carcinomas in patients with and without synchronous liver metastases]. PhD thesis, Sveučilište u Zagrebu.

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Abstract

Protein connexin 43 (Cx43) is an integral part of gap junctions, intercellular channels with important role in cell growth regulation. In colorectal cancer, loss of Cx43 expression is considered a poor prognostic factor. The aim of our study was to investigate the possible role of Cx43 expression as a biomarker of metastatic potential. Immumohistochemical expression of Cx43 was analyzed in 50 colorectal adenocarcinomas from surgically treated patients of stage pT3N1-2 without metastases (M0) and 50 specimens of same pTN stage from patients with synchronous liver metastases (M1). Additionally, we analyzed Cx43 expression in tumors localized in colon and rectum, cellular localization of Cx43 and correlation of histopathological prognostic factors with Cx43 expression. Reactivity to Cx43 was exclusively cytoplasmic, with no nuclear staining observed. There was no significant difference in cytoplasmic Cx43 expression between M0 and M1 specimens (p=0.817). We observed Cx43 expression in peritumoral stroma in 46% M0 and 74% M1 tumors, respectively (p=0,004). There was no difference in Cx43 expression between colonic and rectal tumors. No correlation of histopathological prognostic factors with Cx43 expression was showed in our study. Our results suggest that stromal, not cytoplasmic expression of Cx43 is the possible indicator of metastatic potential of colorectal adenocarcinoma.

Abstract in Croatian

Bjelančevina koneksin 43 (Cx43) sastavni je dio pukotinskih spojeva, meĎustaničnih kanalića koji imaju važnu ulogu u kontroli staničnog rasta. Smatra se da je gubitak izraženosti Cx43 čimbenik lošije prognoze u bolesnika s kolorektalnim karcinomom. Cilj našeg istraživanja bio je ispitati ulogu stanične izraženosti Cx43 kao potencijalnog biljega metastatskog potencijala. Imunohistokemijska izraženost Cx43 analizirana je u uzorku od 50 adenokarcinoma debelog crijeva operiranih bolesnika stadija pT3N1-2 bez presadnica (M0) i 50 uzoraka istog pTN stadija bolesnika sa sinkronim jetrenim presadnicama (M1). Dodatno je analizirana izraženost Cx43 u tumorima lokaliziranim u kolonu i rektumu i stanična lokalizacija Cx43 te korelacija histopatoloških prognostičkih čimbenika s izraženošću Cx43. Reakcija na Cx43 u tumorskim stanicama bila je isključivo citoplazmatska, dok nuklearna reakcija nije uočena. Nije dokazana statistički značajna razlika u citoplazmatskoj izraženosti Cx43 izmeĎu skupina M0 i M1 (p=0,817). Uočena je izraženost Cx43 u peritumorskoj stromi, i to u 46% M0 uzoraka i 74% M1 uzoraka (p=0,004). Nije naĎena statistički značajna razlika u izraženosti Cx43 u tumorima kolona i rektuma, a nije dokazana niti jasna povezanost izraženosti Cx43 i poznatih patohistoloških prognostičkih čimbenika. Naši rezultati ukazuju na ulogu stromalne, a ne citoplazmatske izraženosti Cx43 kao mogućeg pokazatelja metastatskog potencijala adenokarcinoma debelog crijeva.

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