The relationship between methylenetetrahydrofolate reductase C677T gene polymorphism and diabetic nephropathy in Croatian type 2 diabetic patients [Povezanost polimirfizma C677T gena za metilentetrahidrofolat reduktazu i dijabetičke nefropatije u bolesnika sa šećernom bolesti tip 2 u Hrvatskoj]

Gojo Tomić, Nives and Marušić, Srećko and Božikov, Velimir and Kušec, Rajko and Bačić-Vrca, Vesna and Tadić, Mario (2013) The relationship between methylenetetrahydrofolate reductase C677T gene polymorphism and diabetic nephropathy in Croatian type 2 diabetic patients [Povezanost polimirfizma C677T gena za metilentetrahidrofolat reduktazu i dijabetičke nefropatije u bolesnika sa šećernom bolesti tip 2 u Hrvatskoj]. Collegium Antropologicum, 37 (3). pp. 789-793. ISSN 0350-6134

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Abstract

Methylenetetrahydrofolate reductase (MTHFR) polymorphism has been shown to be associated with the development of diabetic nephropathy in many ethnic groups. In this study, we examined the correlation between MTHFR C677T polymorphism and microalbuminuria in patients with diabetes mellitus type 2 in Croatian patients. 85 patients with diabetes mellitus type 2 were recruited. Patients were classified into two groups--with and without diabetic nephropathy according to urinary albumin excretion rate in urine collected during 24 hours. The C677T genotype was determined by real-time PCR analysis. The genotype frequencies were CC 36.5%, CT 42.3% and TT 21.2% in diabetic patients without nephropathy versus CC 39.4%, CT 45.4% and TT 15.2% in those with nephropathy. There was no statistically significant difference in allele distribution between patients with nephropathy and those without (p = 0.788). Our study did not show a correlation between mutations in the MTHFR gene and diabetic nephropathy in Croatian patients. Diabetic nephropathy is influenced by multiple risk factors which can modify the importance of MTHFR polymorphism in its development.

Abstract in Croatian

Polimorfizam gena za metilentetrahidrofolat reduktazu (MTHFR) je povezan s razvojem dijabetičke nefropatije u mnogim etničkim skupinama.U ovoj studiji istraživali smo povezanost između polimorfizma C677T gena za MTHFR i mikroalbuminurije u bolesnika sa šećernom bolesti tip 2 u hrvatskih bolesnika. Osamdeset pet bolesnika sa šećernom bolesti je obrađivano i svrstano u dvije skupine – sa dijabetičkom nefropatijom i bez nje, ovisno o vrijednostima izlučenog albumina u 24-satnom urinu. Mutacija C677T gena za MTHFR određena je metodom izolacije DNA i alel specifične lančane reakcije polimeraze (PCR). Raspodjela genotipova u skupini bolesnika s normoalbuminurijom bila je: CC 36,5%, CT 42,3% i TT 21,2%, dok je u skupini bolesnika sa mikroalbuminurijom bila: CC 39,4%, CT 45,4% i TT 15,2%. Nije bilo statistički značajne razlike među grupama u distribuciji C677T genotipova (p=0,788). Naše istraživanje nije pokazalo povezanost između mutacije gena za metilentetrahidrofolat reduktazu i dijabetičke nefropatije u hrvatskih bolesnika. Mnogostruki čimbenici mogu modificirati značajnost utjecaja polimorfizma MTHFR na nastanak dijabetičke nefropatije.

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