Expression of chemokine receptor CX3CR1 in infants with respiratory syncytial virus bronchiolitis

Cepika, Alma-Martina and Gagro, Alenka and Baće, Ana and Tješić-Drinković, Dorian and Kelečić, Jadranka and Baričić-Voskresensky, Tamara and Matić, Mladen and Draženović, Vladimir and Marinić, Igor and Mlinarić-Galinović, Gordana and Tješić-Drinković, Duška and Vrtar, Zvonimir and Rabatić, Sabina (2008) Expression of chemokine receptor CX3CR1 in infants with respiratory syncytial virus bronchiolitis. Pediatric Allergy and Immunology, 19 (2). pp. 148-56. ISSN 0905-6157

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Respiratory syncytial virus (RSV) glycoprotein G mimics fractalkine, a CX(3)C chemokine, which mediates chemotaxis of leukocytes expressing its receptor, CX(3)CR1. The aim of this study was to examine the relationship between RSV infection and expression of perforin and IFN-gamma in CX(3)CR1-expressing peripheral blood CD8(+) T cells. Samples were collected from infants with RSV bronchiolitis, both in the acute and convalescence phase (n = 12), and from their age- and sex-matched healthy controls (n = 15). Perforin expression and IFN-gamma secretion in CX(3)CR1(+) CD8(+) T cells were assessed by four-color flow cytometry. The NF-kappaB p50 and p65 subunit levels were also determined as markers of RSV-induced inflammation. Study results showed perforin and CX(3)CR1 expression to be significantly lower in the convalescent phase of infected infants than in healthy controls. There was no significant difference in IFN-gamma secretion and NF-kappaB binding activity between two time-points in RSV-infected infants, or when compared with healthy controls. Infants with prolonged wheezing had lower acute-phase CX(3)CR1 levels in peripheral blood. These data indicate existence of an event persisting after acute RSV infection that is able to modulate effector functions of cytotoxic T cells, and also link disease severity with CX(3)CR1 expression.

Item Type: Article
MeSH: Acute Disease ; Biological Markers/metabolism ; Bronchiolitis, Viral/blood ; Bronchiolitis, Viral/immunology ; CD8-Positive T-Lymphocytes/immunology ; CD8-Positive T-Lymphocytes/secretion ; Cell Nucleus/metabolism ; Convalescence ; Enzyme-Linked Immunosorbent Assay ; Female ; Flow Cytometry ; Humans ; Infant ; Infant, Newborn ; Interferon-gamma/biosynthesis ; Interferon-gamma/secretion ; Male ; Perforin/biosynthesis ; Receptors, Chemokine/biosynthesis ; Respiratory Syncytial Virus Infections/blood ; Respiratory Syncytial Virus Infections/immunology
Departments: Katedra za pedijatriju
Katedra za bakteriologiju, virologiju i parasitologiju
Depositing User: Marijan Šember
Status: Published
Cepika, Alma-MartinaUNSPECIFIED
Tješić-Drinković, DorianUNSPECIFIED
Kelečić, JadrankaUNSPECIFIED
Baričić-Voskresensky, TamaraUNSPECIFIED
Draženović, VladimirUNSPECIFIED
Mlinarić-Galinović, GordanaUNSPECIFIED
Tješić-Drinković, DuškaUNSPECIFIED
Vrtar, ZvonimirUNSPECIFIED
Rabatić, SabinaUNSPECIFIED
Date: March 2008
Date Deposited: 05 Mar 2010
Last Modified: 23 Sep 2011 16:11
Subjects: /
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