The frequency of micronuclei in peripheral blood lymphocytes and buccal exfoliated cells in women with cervical cancer

Gashi, Goneta (2018) The frequency of micronuclei in peripheral blood lymphocytes and buccal exfoliated cells in women with cervical cancer. PhD thesis, Sveučilište u Zagrebu.

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Abstract

A biological marker is an important aspect of the diagnosis, prognosis and risk assessment of a disease. The aim of this study was the evaluation of genomic instability in patients with cervical lesions. The genetic damages were investigated in 100 subjects: patients with low grade squamous intraepithelial lesions (LSIL; n=20), patients with high grade squamous intraepithelial lesions (HSIL; n=20) patients with invasive squamous cervical cancer (SCC; n=20) and healthy women (n=20) with cytokinesis-block micronucleus cytome (CBMN cyt) assay in peripheral blood lymphocytes (PBL), and buccal micronucleus assay in buccal exfoliated cells (BEC), in order to assess the frequency of micronucleus (MN) in PBL and frequency of MN in BEC as well as the frequency of other nuclear anomalies such as nucleoplasmic bridges (NPBs) and nuclear bunds (NBUDs) in PBL. The frequency of MN in BEC, MN in PBL, NPB in PBL and NBUD in PBL were significantly higher (p< 0.001), in patients compared to control. Pearson’s correlation revealed a statistically significant strong positive correlation between variables in patients groups (p<0.001). Although larger studies are needed, our data support the predictive value of MN, NPB and NBUD as biomarkers of genomic instability for evaluation of risk level of cervical cancer diseases.

Abstract in Croatian

Biološki marker predstavlja važan dio u dijagnostici, prognozi i procjeni rizika za određenu bolest. Cilj rada bio je procijeniti genomsku nestabilnost kod pacijentica sa cervikalnim lezijama. Genetska oštećenja su istraživana kod 100 žena /20 bolesnica sa skvamoznom intraepitelnom lezijom niskog stupnja (LSIL), 40 bolesnica sa skvamoznom intraepitelnom lezijom visokog stupnja (HSIL), 20 bolesnica sa invazivnim rakom vrata maternice (SCC), 20 žena bez bolesti, kao kontrolna skupina/, primjenom testa blokiranja citokineze mikronuklearnog citoma (engl.cytokinesis-block micronucleus cytome - CBMN cyt) na limfocitima periferne krvi (PBL) te bukalnog mikronuklearnog testa na eksfoliranim bukalnim stanicama (BEC). U radu se takođe istražila učestalost drugih nuklearnih anomalija kao što s nukleoplazmatski mostovi (NPB) i nuklearni pupoljci (NBUD) u PBL. Učestalost MN u BEC, MN u PBL, NPB u PBL i NBUD u PBL značajno je viša (p< 0.001) kod bolesnica nego u kontroloj skupini zdravih žena. Pearsonova korelacija upućuje na jaku pozitivnu povezanost između varijabli u skupinama pacijentica, što je statistički značajno (p<0.001). Premda je potrebno daljnje istraživanje na većem uzorku, naši rezultati podržavaju mišljenje da prediktivne vrijednosti MN, NPB i NBUD predstavljaju biomarkere genomske nestabilnosti za procjenu razine rizika i za nastanak raka vrata maternice.

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