Repozitorij Medicinskog fakulteta Sveučilišta u Zagrebu

Expression of Ki-67, P53 and progesterone receptors in uterine smooth muscle tumors. Diagnostic value

Petrović, Davor and Babić, Damir and Ilić Forko, Jadranka and Martinac, Ivana (2010) Expression of Ki-67, P53 and progesterone receptors in uterine smooth muscle tumors. Diagnostic value. Collegium Antropologicum, 34 (1). pp. 93-7. ISSN 0350-6134

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    Croatian abstract

    EKSPRESIJA KI-67, P53 I PROGESTERONSKIH RECEPTORA U GLATKOMIŠIĆNIM TUMORIMA MATERNICE. DIJAGNOSTIČKA VRIJEDNOST ----- Cilj rad je istražiti ekspresiju Ki-67, p53 i progesteronskih receptora (PR) u leiomiomima (LM), glatkomišićnim tumorima nepoznata zloćudnog potencijala (STUMP) i leiomiosarkomima (LMS), te utvrditi moguću korisnost ta tri promatrana parametra u olakšavanju razlikovanja LM, STUMP i LMS. Učinjena je retrospektivna studija 51 glatkomišićnog tumora (16 LM, 18 STUMP, 17 LMS), tehnički pogodnih za analizu, dijagnosticiranih u Zavodu za ginekološku i perinatalnu patologiju Kliničkog bolničkog centra Zagreb u razdoblju 2002.–2007. godine. Izvršena je imunohistokemijska analiza ekspresije Ki-67, p53 i PR. Statistička analiza učinjena je primjenom ne-parametrijskih metoda analize varijance Kruskal-Walis-ovim testom. U svim LM nije nađena ekspresija Ki-67, ekspresija Ki-67 viša od 5% nađena je u 12/18 STUMP i 10/17 LMS. Značajna je razlika u ekspresiji Ki-67 između LM i STUMP (p=0,000) te između LM i LMS (p=0,000). Nije nađena ekspresija p53 u LM. Ekspresija p53 nađena je u 7/17 LMS i 5/18 STUMP. Značajna je razlika u ekspresiji p53 između LM i LMS (p=0,002) te između LM i STUMP (p=0,006). Ekspresija PR nađena je u 16/16 LM i 18/18 STUMP, dok 10/17 LMS nije pokazalo nikakvu ekspresiju PR. Značajna je razlika u ekspresiji PR između LM i LMS (p=0,018) te između STUMP i LMS (p=0,004). Nalazi naše studije u suglasju s rezultatima drugih studija su korisna novost u utvrđivanju novih dijagnostičkih kriterija i parametara za olakšavanje postavljanja dijagnoze u nejasnim slučajevima ova tri entiteta. Imunohistokemijsko utvrđivanje ekspresije Ki-67, p53 i PR, te panel rezultata njihove ekspresije olakšava dijagnozu u pojedinim nejasnim slučajevima.

    English abstract

    Aim was to investigate expression of Ki-67, P53 and progesterone receptors (PR) in leiomyomas (LM), smooth muscle tumors of uncertain malignant potential (STUMP) and leiomyosarcomas (LMS) and to establish possible usefulness of these three parameters in distinguishing between LM and STUMP, and STUMP and LMS. Retrospective study of 51 uterine smooth muscle neoplasm (16 LM, 18 STUMP, 17 LMS) technically acceptable for analyses from years 2002–2007 from Department of Gynecological and Prenatal Pathology, University Hospital Center Zagreb, Croatia. Immunohistochemical analysis of Ki-67, P53 and PR expression was performed. Every nuclei stained brown, regardless of shade intensivity, was considered positive. The interpretation of immunohistochemical staining was expressed as number of positive cells in 100 cell count in most active area of the slide. Non-parametric analysis of variance Kruskal-Walis test was performed. Ki-67 expression was negative in all LM and higher than 5% in 12/18 STUMP and 10/17 LMS. Significant differences were observed between LM and STUMP expression for Ki-67 (p=0.000), and LM and LMS expression for Ki-67 (p=0.000).There was no expression of P53 in LM, expression of P53 was found in 7/17 LMS and 5/18 STUMP. Expression of P53 was significant between LM and LMS (p=0.002), and between LM and STUMP (p=0.006). Expression of PR was found in 16/16 LM and 18/18 STUMP, 10/17 LMS did not show PR expression. Expression of PR was significant between LM and LMS (p=0.018) and STUMP and LMS (p=0.004). The findings of our study in concordance with other study results are helpful information establishing more diagnostic criteria and parameters for diagnosis in doubtful cases between three entities. Immunoassaying for Ki-67, P53 and PR are such parameters. The panel of their expression in specific case eases diagnosis.

    Item Type: Article
    MeSH: Cell Count ; Female ; Humans ; Immunohistochemistry ; Ki-67 Antigen/metabolism ; Leiomyoma/metabolism ; Leiomyoma/pathology ; Leiomyosarcoma/metabolism ; Leiomyosarcoma/pathology ; Muscle, Smooth/metabolism ; Muscle, Smooth/pathology ; Myometrium/metabolism ; Myometrium/pathology ; Receptors, Progesterone/metabolism ; Tumor Markers, Biological/metabolism ; Tumor Suppressor Protein p53/metabolism ; Uterine Neoplasms/metabolism ; Uterine Neoplasms/pathology
    Divisions: Katedra za patologiju
    Depositing User: Marijan Šember
    Status: Published
    Creators:
    CreatorsEmail
    Petrović, Davor
    Babić, Damir
    Ilić Forko, Jadranka
    Martinac, Ivana
    Date: March 2010
    Date Deposited: 01 Jun 2010
    Last Modified: 23 Sep 2011 18:11
    Subjects: /
    Related URLs:
      URI: http://medlib.mef.hr/id/eprint/802

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