Morphometry of tumor cells in different grades and types of breast cancer

Prvulović, Ivana and Kardum-Skelin, Ika and Susterčić, Dunja and Jakić-Razumović, Jasminka and Manojlović, Spomenka (2010) Morphometry of tumor cells in different grades and types of breast cancer. Collegium Antropologicum, 34 (1). pp. 99-103. ISSN 0350-6134

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Abstract

The aim of the study was to compare morphometric characteristics of different types and grades of breast cancer. Morphometric analysis was performed using the SFORM software (Vamstec, Zagreb) on the May-Grünwald-Giemsa stained fine needle aspiration cytology (FNAC) breast tissue specimens. The study included 42 patients diagnosed with breast carcinoma by breast smear FNAC at Merkur University Hospital during the 2001–2005 period. Postoperative tumor histopathology and semi-quantitative tumor grading by the method of Elston and Ellis1 showed invasive ductal carcinoma grade I in 10, invasive ductal carcinoma grade II in 9, invasive ductal carcinoma grade III in 13, and invasive lobular carcinoma in 13 patients, the latter also including a subtype of invasive tubulolobular carcinoma. The following parameters were assessed by use of Statistica 7.1 and c 2-test: tumor area, circumference, maximal radius, minimal radius, convexity, length, width, elongation, nucleus/cytoplasm ratio, and shape factor. Morphometric analysis yielded statistically significant differences among all study groups (p<0.001). Morphometric parameters showed significant individual correlation with tumor type and grade, whereby the area, convexity and circumference were most significant at both nuclear and cellular level.

Abstract in Croatian

MORFOMETRIJA TUMORSKIH STANICA KOD RAZLIČITIH GRADUSA I TIPOVA KARCINOMA DOJKE ----- Cilj istraživanja bio je usporediti morfometrijske karakteristike karcinoma dojke različitog subtipa i stupnja (gradusa) diferenciranosti. Morfometrijska analiza provedena je na citološkim razmazima punktata dojke obojenim po metodi May-Grünwald-Giemsa (MGG) programom SFORM (Vamstec, Zagreb). Istraživanjem su obuhvaćene 42 bolesnice kod kojih je dijagnoza karcinoma dojke postavljena na uzorcima dobivenim citološkom punkcijom dojke u Kliničkoj bolnici Merkur u razdoblju od 2001. do 2005. godine. Postoperativno je učinjena patohistološka verifikacija te određivanje gradusa tumora semi-kvantitativnom metodom prema Elstonu i Ellisu1. Kod 10 bolesnica radilo se o invazivnom duktalnom karcinomu gradusa I (IDC I), kod 9 bolesnica o invazivnom duktalnom karcinomu gradusa II (IDC II), kod 13 bolesnica o invazivnom duktalnom karcinomu gradusa III (IDC III), a kod 10 bolesnica postavljena je dijagnoza invazivnog lobularnog karcinoma (ILC) u koji je uključen i invazivni tubulo-lobularni karcinom (TLC) kao njegov podtip. Istraživani su slijedeći parametri: površina, opseg, maksimalni radijus, minimalni radijus, konveksitet, dužina, širina, izduženost, omjer jezgra/citoplazma i faktor oblika. Korištena je statistika 7,1, c2-test. Morfometrijska je analiza u ovom istraživanju pokazala statistički značajne razlike, (p<0,001), između svih ispitivanih grupa. Morfometrijski su parametri, primjenjeni pojedinačno, bili značajno povezani sa tipom i gradusom tumora, a najznačajniji su parametri, i za jezgru i za stanicu, bili: površina, konveksitet i opseg.

Item Type: Article
MeSH: Biopsy, Fine-Needle ; Breast Neoplasms/pathology ; Carcinoma, Ductal, Breast/pathology ; Carcinoma, Lobular/pathology ; Female ; Humans ; Image Processing, Computer-Assisted ; Severity of Illness Index ; Software
Departments: Katedra za patologiju
Depositing User: Marijan Šember
Status: Published
Creators:
CreatorsEmail
Prvulović, IvanaUNSPECIFIED
Kardum-Skelin, IkaUNSPECIFIED
Susterčić, DunjaUNSPECIFIED
Jakić-Razumović, JasminkaUNSPECIFIED
Manojlović, SpomenkaUNSPECIFIED
Date: March 2010
Date Deposited: 31 May 2010
Last Modified: 23 Sep 2011 16:11
Subjects: /
Related URLs:
    URI: http://medlib.mef.hr/id/eprint/801

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