Repozitorij Medicinskog fakulteta Sveučilišta u Zagrebu

Alterations of FHIT and P53 genes in keratocystic odontogenic tumor, dentigerous and radicular cyst

Malčić, Ana and Jukić, Silvana and Anić, Ivica and Pavelić, Božidar and Kapitanović, Sanja and Krušlin, Božo and Pavelić, Krešimir (2008) Alterations of FHIT and P53 genes in keratocystic odontogenic tumor, dentigerous and radicular cyst. Journal of Oral Pathology & Medicine, 37 (5). pp. 294-301. ISSN 0904-2512

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    English abstract

    BACKGROUND: The purpose of this study was to determine fragile histidine triad (FHIT) and p53 protein expression, and to analyze FHIT and p53 gene status in keratocystic odontogenic tumor (KOT), dentigerous cysts (DC) and radicular cysts (RC). ----- METHODS: The methods used were immunohistochemistry and molecular genetic methods including loss of heterozygosity (LOH) and gene sequencing. ----- RESULTS: FHIT protein expression was different among groups. Aberrant expression was the highest in KOT, then in RC and DC. p53 protein expression was different among groups. LOH in paraffin-embedded specimens was detected in 22.6% and 12.9% for FHIT and p53 respectively. Mutation of p53 gene at codon 237 was observed in only two specimens (one KOT and one DC). Of the six frozen specimens, three exhibited FHIT gene LOH (two RC and one KOT). KOT showed loss of exons 6-7 at FHIT locus and mutation at codon 237 at p53 locus, but this could be a chance result. ----- CONCLUSION: Aberrations of FHIT and p53 genes/proteins could be considered markers responsible for the development of odontogenic lesions.

    Item Type: Article
    MeSH: Acid Anhydride Hydrolases/genetics ; Adolescent ; Adult ; Aged ; Apoptosis ; Cell Proliferation ; DNA Mutational Analysis ; DNA, Neoplasm/analysis ; Dentigerous Cyst/genetics ; Dentigerous Cyst/metabolism ; Female ; Genes, Tumor Suppressor ; Genes, p53/genetics ; Humans ; Immunohistochemistry ; Jaw Cysts/genetics ; Jaw Cysts/metabolism ; Jaw Neoplasms/genetics ; Jaw Neoplasms/metabolism ; Loss of Heterozygosity ; Male ; Middle Aged ; Neoplasm Proteins/genetics ; Odontogenic Tumors/genetics ; Odontogenic Tumors/metabolism ; Radicular Cyst/genetics ; Radicular Cyst/metabolism
    Divisions: Katedra za patologiju
    Depositing User: Marijan Šember
    Status: Published
    Malčić, Ana
    Jukić, Silvana
    Anić, Ivica
    Pavelić, Božidar
    Kapitanović, Sanja
    Krušlin, Božo
    Pavelić, Krešimir
    Date: May 2008
    Date Deposited: 11 Mar 2010
    Last Modified: 23 Sep 2011 18:11
    Subjects: /
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