Repozitorij Medicinskog fakulteta Sveučilišta u Zagrebu

Loss of heterozygosity of selected tumor suppressor genes in human testicular germ cell tumors

Vladušić, Tomislav and Hrašćan, Reno and Vrhovac, Ivana and Krušlin, Božo and Gamulin, Marija and Grgić, Mislav and Pećina-Šlaus, Nives and Franekić Čolić, Jasna (2010) Loss of heterozygosity of selected tumor suppressor genes in human testicular germ cell tumors. Pathology, Research and Practice, [Epub . ISSN 0344-0338

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    English abstract

    Human testicular germ cell tumors (TGCTs) are histologically heterogenous neoplasms with a variable malignant potential. Two main groups of germ cell tumors occur in men: seminomas and nonseminomas. In the present study, a set of four tumor suppressor genes was investigated in testicular cancers. CDH1, APC, p53, and nm23-H1 genes were tested for loss of heterozygosity (LOH). Thirty-eight testicular germ cell tumors (17 seminomas and 21 nonseminomas) were analyzed by PCR using restriction fragment length polymorphism or the dinucleotide/tetranucleotide repeat polymorphism method. An allelic loss of p53 at exon 4 was detected in five nonseminomas, whereas LOH of p53 at intron 6 occurred in one of the seminoma and two of the nonseminoma samples. Allelic losses of the APC gene were present in three seminomas and one nonseminoma, whereas one seminoma and three nonseminomas showed LOH of CDH1. The analysis of allelic losses showed no common structural genetic alterations in tumor tissues, although a different pattern of LOH was observed between the two main histological groups of TGCTs.

    Item Type: Article
    Divisions: Katedra za medicinsku biologiju
    Katedra za radiologiju i opću kliničku onkologiju
    Katedra za patologiju
    Depositing User: Marijan Šember
    Status: Published
    Vladušić, Tomislav
    Hrašćan, Reno
    Vrhovac, Ivana
    Krušlin, Božo
    Gamulin, Marija
    Grgić, Mislav
    Pećina-Šlaus, Nives
    Franekić Čolić, Jasna
    Date: 19 January 2010
    Date Deposited: 09 Feb 2010
    Last Modified: 23 Sep 2011 18:11
    Subjects: /
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