Repozitorij Medicinskog fakulteta Sveučilišta u Zagrebu

Variants of ESR1, APOE, LPL and IL-6 loci in young healthy subjects: association with lipid status and obesity

Sertić, Jadranka and Juričić, Ljiljana and Ljubić, Hana and Božina, Tamara and Lovrić, Jasna and Markeljević, Jasenka and Jelaković, Bojan and Merkler, Marijan and Reiner, Željko (2009) Variants of ESR1, APOE, LPL and IL-6 loci in young healthy subjects: association with lipid status and obesity. BMC Research Notes, 2 (203). ISSN 1756-0500

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    English abstract

    Findings BMI was increased (>25) in 22% of young healthy subjects. Increased cholesterol values (>5.0 mmol/L) were found in 23% of subjects, LDL-C (>3.0 mmol/L) in 23%, triglycerides (>1.7 mmol/L) in 11% of subjects. We found statistically significant differences in subjects' weight (p = 0.015), BMI (p = 0.023), and waist-hip ratio (WHR) (p = 0.015) in regard to their diet type; subjects with Mediterranean diet had the lowest values compared to those on continental and mixed diet. Significant associations were found for: LPL genetic polymorphic variant and abdominal obesity (p = 0.013), APO epsilon4 allele and hypercholesterolemia (p = 0.003), and ESR1-TA long allele and hypercholesterolemia (p = 0.011). ----- Background Human obesity is a multifactorial syndrome influenced also by genetic factors. Among gene variants found to be involved in body weight regulation and development of obesity, particular attention has been paid to polymorphisms in genes associated with obesity-related metabolic disorders. We explored the association of genetic polymorphisms of: estrogen receptor alpha (ESR1-TA repeats); interleukin-6 (IL-6 G-174C); apolipoprotein E (APO epsilon2, epsilon3, epsilon4); lipoprotein lipase Pvu II (LPL P+/-), with clinical variables: gender, age, body mass index (BMI), diet type and biological variables: triglycerides, cholesterol, HDL-C, LDL-C, CRP, homocysteine, urate, and glucose in 105 healthy young subjects (20-35 yrs) of Croatian origin. ----- Methods Genotyping of IL-6, LPL was performed by PCR-RFLP, of APOE by real-time PCR, and of ESR1 by PCR and capillary electrophoresis. Association analyses were performed of alleles and genotypes with biological variables. ----- Conclusion ESR-1, LPL, and APO E genetic polymorphic variants could represent predictive genetic risk markers for obesity-related metabolic disorders in young healthy subjects. Mediterranean type of diet is also an important protective factor against abdominal obesity.

    Item Type: Article
    Divisions: Katedra za medicinsku kemiju, biokemiju i kliničku kemiju
    Katedra za internu medicinu
    Depositing User: Marijan Šember
    Status: Published
    Sertić, Jadranka
    Juričić, Ljiljana
    Ljubić, Hana
    Božina, Tamara
    Lovrić, Jasna
    Markeljević, Jasenka
    Jelaković, Bojan
    Merkler, Marijan
    Reiner, Željko
    Date: 05 October 2009
    Date Deposited: 20 Jan 2010
    Last Modified: 23 Sep 2011 18:11
    Subjects: /
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