Repozitorij Medicinskog fakulteta Sveučilišta u Zagrebu

Persistent accumulation of 4-hydroxynonenal-protein adducts in gastric mucosa after Helicobacter pylori eradication.

Cherkas, Andriy and Yelisyeyeva, Olha and Semen, Khrystyna and Žarković, Kamelija and Kaminskyy, Danylo and Čipak Gašparović, Ana and Jaganjac, Morana and Lutsyk, Alexander and Waeg, Georg and Žarković, Neven (2009) Persistent accumulation of 4-hydroxynonenal-protein adducts in gastric mucosa after Helicobacter pylori eradication. Collegium antropologicum, 33 (3). pp. 815-821. ISSN 0350-6134

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    Croatian abstract

    POVEĆANO NAKUPLJANJE 4-HIDROKSINONENAL-PROTEINSKIH KONJUGATA U ŽELUČANOJ SLUZNICI ZAOSTAJE NAKON IZLJEČENJA HELICOBACTER PYLORI ----- Dosadašnja istraživanja pokazala su da bi oksidacijski stres uzrokovan bakterijom Helicobacter pylori, zajedno sa smanjenom antioksidacijskom zaštitom domaćina, mogao igrati značajnu ulogu u patogenezi želučanog ulkusa. U prijašnjem radu dokazali smo izvornim monoklonskim protutijelima slabi pozitivitet 4-hidroksinonenala (HNE), glavnog bioaktivnog biljega lipidne peroksidacije, u fiziološkim uvjetima u zdravoj ljudskoj želučanoj sluznici. Pozitivitet HNEa pojačavao se u slučaju infekcije s H. pylori kao i kod peptičnog ulcua. Obzirom na fiziološku prisutnost HNEa s jedne strane i visoke učestalosti bolesti vezane uz H. pylori s druge strane, procjena oksidacijskog stresa nakon tretmana mogla bi biti važan progostički parametar. U ovom radu praćena je imunohistokemijska distribucija HNE-proteinskih konjugata u želučanoj sluznici kod 21 pacijenta sa ulcusom dvanaesnika (DPU) izazvanim H. pylori prije liječenja i mjesec dana poslije liječenja. Uočeno povećanje i preraspodjela HNE-pozitiviteta u jezgri žljezdanih stanica u tijelu želuca ukazuju na povećanje oksidacijskog stresa nakon tretmana te time pruža mogućnost adjuvantnog antioksidacijskog tretmana za zaštitu sluznice želuca od razvoja oksidacijskog stresa i lipidne peroksidacije u i nakon infekcije s H. pylori. Nakon izliječenja H. pylori, histološki pokazatelji upale su drastično sniženi, no količina HNE- proteinskih konjugata je i dalje povišena u antrumu, te dodatno povišena u tijelu želuca.

    English abstract

    Recent studies indicate that oxidative stress caused by Helicobacter pylori and insufficient host antioxidant defense could play important role in pathogenesis of gastrointestinal ulcerations. By specific monoclonal antibodies we have detected weak presence of the major lipid peroxidation bioactive marker 4-hydroxynonenal (HNE) in healthy human gastric mucosa, which strongly increased in case of H. pylori-associated peptic ulcer. Considering physiological presence of HNE on one hand, and high prevalence of H. pylori associated disorders on the other, evaluation of oxidative stress after treatment is important. Therefore, in current study immunohistochemical accumulation and distribution of HNE-protein adducts in gastric mucosa was evaluated with 21 patients having H. pylori-associated duodenal peptic ulcer (DPU) before and one month after eradication of H. pylori. Although dramatic decrease in histological manifestations of inflammation was demonstrated after eradication of H. pylori, initially high immunopositivity for the HNE-protein adducts remained elevated in antrum and even increased in stomach corpus. The observed accumulation and redistribution to higher grades of HNE-immunopositivity in nuclei of glandular cells in gastric corpus indicate augmentation of oxidative stress after treatment and open possibilities for adjuvant antioxidant treatments to protect gastric mucosa from progressive oxidative stress after eradication of H. pylori infection.

    Item Type: Article
    Divisions: Katedra za patologiju
    Depositing User: Marijan Šember
    Status: Published
    Creators:
    CreatorsEmail
    Cherkas, Andriy
    Yelisyeyeva, Olha
    Semen, Khrystyna
    Žarković, Kamelija
    Kaminskyy, Danylo
    Čipak Gašparović, Ana
    Jaganjac, Morana
    Lutsyk, Alexander
    Waeg, Georg
    Žarković, Neven
    Date: September 2009
    Date Deposited: 13 Nov 2009
    Last Modified: 23 Sep 2011 18:11
    Subjects: /
    Related URLs:
      URI: http://medlib.mef.hr/id/eprint/690

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