FT-IR spectroscopy of lipoproteins – a comparative study

Krilov, Dubravka and Balarin, Maja and Kosović, Marin and Gamulin, Ozren and Brnjas-Kraljević, Jasminka (2009) FT-IR spectroscopy of lipoproteins – a comparative study. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 73 (4). pp. 701-706. ISSN 1386-1425

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Official URL: https://doi.org/10.1016/j.saa.2009.03.015

Abstract

FT-IR spectra, in the frequency region 4000 – 600 cm-1, of four major lipoprotein classes: very low density lipoprotein (VLDL), low density lipoprotein (LDL) and two subclasses of high density lipoproteins (HDL2 and HDL3) were analyzed to obtain their detailed spectral characterization. Information about the protein domain of particle was obtained from the analysis of amide I band. The procedure of decomposition and curve fitting of this band confirms the data already known about the secondary structure of two different apolipoproteins: apo A-I in HDL2 and HDL3 and apo B-100 in LDL and VLDL. For information about the lipid composition and packing of the particular lipoprotein the well expressed lipid bands in the spectra were analyzed. Characterization of spectral details in the FT-IR spectrum of natural lipoprotein is necessary to study the influence of external compounds on its structure.

Item Type:

Article

MeSH:

Apolipoproteins/analysis ; Apolipoproteins/chemistry ; Humans ; Lipids/analysis ; Lipids/chemistry ; Lipoproteins/analysis ; Lipoproteins/chemistry ; Lipoproteins, HDL2/analysis ; Lipoproteins, HDL2/chemistry ; Lipoproteins, HDL3/analysis ; Lipoproteins, HDL3/chemistry ; Lipoproteins, LDL/analysis ; Lipoproteins, ; LDL/chemistry ; Lipoproteins, VLDL/analysis ; Lipoproteins, VLDL/chemistry ; Protein Structure, Secondary ; Spectroscopy, Fourier Transform Infrared/methods

Departments:

Katedra za fiziku i biofiziku

Depositing User:

Marijan Šember

Status:

Published

Creators: