Repozitorij Medicinskog fakulteta Sveučilišta u Zagrebu

Increased bone resorption and osteopenia are a part of the lymphoproliferative phenotype of mice with systemic over-expression of interleukin-7 gene driven by MHC class II promoter

Salopek, Daniela and Grčević, Danka and Katavić, Vedran and Kovačić, Nataša and Lukić, Ivan Krešimir and Marušić, Ana (2008) Increased bone resorption and osteopenia are a part of the lymphoproliferative phenotype of mice with systemic over-expression of interleukin-7 gene driven by MHC class II promoter. Immunology Letters, 121 (2). pp. 134-139. ISSN 0165-2478 (Print) 1879-0542 (Electronic)

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    English abstract

    Mice with interleukin (IL)-7 transgene under the control of E(alpha) promoter over-express IL-7 in MHC class II-positive cells and develop specific immune phenotype, marked by an increase in CD45R(+) cells in both the bone marrow and peripheral blood. We show that IL-7 transgenic mice have a bone phenotype characterized by an age-related loss of trabecular bone in both axial and long bones. Osteopenia was the result of increased number of active osteoclasts on the surface of trabecular bone. Furthermore, IL-7 transgenic mice showed increased osteoclastic but unchanged osteoblastic potential of the bone marrow in vitro. IL-7 over-expression also created osteoclastogenic microenvironment within the bone marrow which promoted the commitment of precursors towards the osteoclast lineage. These findings are important for immunological disturbances where IL-7 is involved and where alterations in the immune system are accompanied by changes in bone metabolism, such as multiple myeloma, rheumatoid arthritis and postmenopausal osteoporosis.

    Item Type: Article
    MeSH: Age Factors ; Animals ; Antigens, CD45/blood ; Antigens, CD45/genetics ; Antigens, CD45/immunology ; Bone Diseases, Metabolic/blood ; Bone Diseases, Metabolic/genetics ; Bone Diseases, Metabolic/immunology ; Bone Marrow/immunology ; Bone Marrow/metabolism ; Bone Resorption/blood ; Bone Resorption/genetics ; Bone Resorption/immunology ; Cell Differentiation ; Cell Lineage ; Cell Proliferation ; Genes, MHC Class II/genetics ; Genes, MHC Class II/immunology ; Interleukin-7/biosynthesis ; Interleukin-7/genetics ; Interleukin-7/immunology ; Lymphocytes/metabolism ; Mice ; Mice, Inbred C57BL ; Mice, Transgenic ; Osteoclasts/metabolism ; Promoter Regions, Genetic/genetics
    Divisions: Katedra za fiziologiju i imunologiju
    Katedra za anatomiju i kliničku anatomiju
    Katedra za radiologiju i opću kliničku onkologiju
    Depositing User: Boris Čičovački
    Status: Published
    Creators:
    CreatorsEmail
    Salopek, Daniela
    Grčević, Danka
    Katavić, Vedran
    Kovačić, Nataša
    Lukić, Ivan Krešimir
    Marušić, Ana
    Date: 22 December 2008
    Date Deposited: 30 Sep 2009
    Last Modified: 23 Sep 2011 18:10
    Subjects: /
    Related URLs:
      URI: http://medlib.mef.hr/id/eprint/595

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