Increased bone resorption and osteopenia are a part of the lymphoproliferative phenotype of mice with systemic over-expression of interleukin-7 gene driven by MHC class II promoter

Salopek, Daniela and Grčević, Danka and Katavić, Vedran and Kovačić, Nataša and Lukić, Ivan Krešimir and Marušić, Ana (2008) Increased bone resorption and osteopenia are a part of the lymphoproliferative phenotype of mice with systemic over-expression of interleukin-7 gene driven by MHC class II promoter. Immunology Letters, 121 (2). pp. 134-139. ISSN 0165-2478 (Print) 1879-0542 (Electronic)

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Abstract

Mice with interleukin (IL)-7 transgene under the control of E(alpha) promoter over-express IL-7 in MHC class II-positive cells and develop specific immune phenotype, marked by an increase in CD45R(+) cells in both the bone marrow and peripheral blood. We show that IL-7 transgenic mice have a bone phenotype characterized by an age-related loss of trabecular bone in both axial and long bones. Osteopenia was the result of increased number of active osteoclasts on the surface of trabecular bone. Furthermore, IL-7 transgenic mice showed increased osteoclastic but unchanged osteoblastic potential of the bone marrow in vitro. IL-7 over-expression also created osteoclastogenic microenvironment within the bone marrow which promoted the commitment of precursors towards the osteoclast lineage. These findings are important for immunological disturbances where IL-7 is involved and where alterations in the immune system are accompanied by changes in bone metabolism, such as multiple myeloma, rheumatoid arthritis and postmenopausal osteoporosis.

Item Type: Article
MeSH: Age Factors ; Animals ; Antigens, CD45/blood ; Antigens, CD45/genetics ; Antigens, CD45/immunology ; Bone Diseases, Metabolic/blood ; Bone Diseases, Metabolic/genetics ; Bone Diseases, Metabolic/immunology ; Bone Marrow/immunology ; Bone Marrow/metabolism ; Bone Resorption/blood ; Bone Resorption/genetics ; Bone Resorption/immunology ; Cell Differentiation ; Cell Lineage ; Cell Proliferation ; Genes, MHC Class II/genetics ; Genes, MHC Class II/immunology ; Interleukin-7/biosynthesis ; Interleukin-7/genetics ; Interleukin-7/immunology ; Lymphocytes/metabolism ; Mice ; Mice, Inbred C57BL ; Mice, Transgenic ; Osteoclasts/metabolism ; Promoter Regions, Genetic/genetics
Departments: Katedra za fiziologiju i imunologiju
Katedra za anatomiju i kliničku anatomiju
Katedra za radiologiju i opću kliničku onkologiju
Depositing User: Boris Čičovački
Status: Published
Creators:
CreatorsEmail
Salopek, DanielaUNSPECIFIED
Grčević, DankaUNSPECIFIED
Katavić, VedranUNSPECIFIED
Kovačić, NatašaUNSPECIFIED
Lukić, Ivan KrešimirUNSPECIFIED
Marušić, AnaUNSPECIFIED
Date: 22 December 2008
Date Deposited: 30 Sep 2009
Last Modified: 23 Sep 2011 16:10
Subjects: /
Related URLs:
    URI: http://medlib.mef.hr/id/eprint/595

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