Historic, demographic, and genetic evidence for increased population frequencies of CCR5Delta32 mutation in Croatian Island isolates after lethal 15th century epidemics

Biloglav, Zrinka and Zgaga, Lina and Smoljanović, Mladen and Hayward, Caroline and Polašek, Ozren and Kolčić, Ivana and Vitart, Veronique and Zemunik, Tatijana and Boraska, Vesna and Torlak, Vesela and Mulić, Rosanda and Ropac, Darko and Grković, Ivica and Rudan, Diana and Ristić, Smiljana and Barbalić, Maja and Campbell, Harry and Wright, Alan F. and Rudan, Igor (2009) Historic, demographic, and genetic evidence for increased population frequencies of CCR5Delta32 mutation in Croatian Island isolates after lethal 15th century epidemics. Croatian Medical Journal, 50 (1). pp. 34-42. ISSN 0353-9504 (Print) 1332-8166 (Electronic)

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Abstract

AIM: To assess the frequency of 32 base pair deletion in CCR5 (CCR5Delta32), which has been shown to confer resistance to HIV infection in a homozygous form, in 10 isolated island communities of Dalmatia, Croatia, with different histories of exposure to epidemics during and since the medieval period. ----- METHODS: In 2002, DNA analysis of 100 randomly selected individuals from each of the 10 isolated communities of 5 Croatian islands (Susak, Rab, Vis, Lastovo, and Mljet) showed high levels of 3-generational endogamy, indicating limited gene flow. Five of the communities were decimated by epidemics of unknown cause between 1449-1456, while the other 5 villages remained unaffected. Genotyping of the CCR5 gene was performed using the polymerase chain reaction method with primers flanking the region containing 32-bp deletion. ----- RESULTS: The frequency of CCR5Delta32 in the 5 villages affected by the epidemic was 6.1-10.0%, and 1.0-3.8% in the 5 unaffected villages. The Delta32 mutation was found in 71 of 916 alleles among the individuals from the affected villages (7.5%), and in 24 of 968 alleles in unaffected villages (2.5%, chi(2)=27.3, P<10-6). A previous study in 303 random Croatian blood donors showed the frequency of the CCR5 Delta32 of 7.1% in the general population. The difference remained significant after correcting for population structure using both STRAT and STRUCTURE software and the genomic control test, to ensure results do not arise from the background genetic differences. ----- CONCLUSION: Our results and historical evidence, suggest that the mid-15th century epidemic could have acted as a selection pressure for the CCR5Delta32 mutation.

Item Type: Article
MeSH: Croatia/epidemiology ; Demography ; Gene Frequency ; Genetics, Population ; Geography ; HIV Infections/genetics ; History, 15th Century ; History, 16th Century ; History, 17th Century ; History, 18th Century ; History, Medieval ; Humans ; Plague/epidemiology ; Plague/genetics ; Plague/history ; Polymerase Chain Reaction ; Polymorphism, Genetic ; Receptors, CCR5/genetics
Departments: Katedra za medicinsku statistiku, epidemiologiju i medicinsku informatiku
Depositing User: Boris Čičovački
Status: Published
Creators:
CreatorsEmail
Biloglav, ZrinkaUNSPECIFIED
Zgaga, LinaUNSPECIFIED
Smoljanović, MladenUNSPECIFIED
Hayward, CarolineUNSPECIFIED
Polašek, OzrenUNSPECIFIED
Kolčić, IvanaUNSPECIFIED
Vitart, VeroniqueUNSPECIFIED
Zemunik, TatijanaUNSPECIFIED
Boraska, VesnaUNSPECIFIED
Torlak, VeselaUNSPECIFIED
Mulić, RosandaUNSPECIFIED
Ropac, DarkoUNSPECIFIED
Grković, IvicaUNSPECIFIED
Rudan, DianaUNSPECIFIED
Ristić, SmiljanaUNSPECIFIED
Barbalić, MajaUNSPECIFIED
Campbell, HarryUNSPECIFIED
Wright, Alan F.UNSPECIFIED
Rudan, IgorUNSPECIFIED
Date: February 2009
Date Deposited: 30 Sep 2009
Last Modified: 23 Sep 2011 16:10
Subjects: /
Related URLs:
    URI: http://medlib.mef.hr/id/eprint/590

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