Janjanin, Saša and Djouad, Farida and Shanti, Rabie M. and Baksh, Dolores and Gollapudi, Kiran and Prgomet, Drago and Rackwitz, Lars and Joshi, Arjun S. and Tuan, Rocky S.
Human palatine tonsil: a new potential tissue source of multipotent mesenchymal progenitor cells.
Arthritis Research & Therapy, 10 (4).
ISSN 1478-6362 (Electronic)
INTRODUCTION: Mesenchymal progenitor cells (MPCs) are multipotent progenitor cells in adult tissues, for example, bone marrow (BM). Current challenges of clinical application of BM-derived MPCs include donor site morbidity and pain as well as low cell yields associated with an age-related decrease in cell number and differentiation potential, underscoring the need to identify alternative sources of MPCs. Recently, MPC sources have diversified; examples include adipose, placenta, umbilicus, trabecular bone, cartilage, and synovial tissue. In the present work, we report the presence of MPCs in human tonsillar tissue. ----- METHODS: We performed comparative and quantitative analyses of BM-MPCs with a subpopulation of adherent cells isolated from this lymphoid tissue, termed tonsil-derived MPCs (T-MPCs). The expression of surface markers was assessed by fluorescent-activated cell sorting analysis. Differentiation potential of T-MPCs was analyzed histochemically and by reverse transcription-polymerase chain reaction for the expression of lineage-related marker genes. The immunosuppressive properties of MPCs were determined in vitro in mixed lymphocyte reactions. ----- RESULTS: Surface epitope analysis revealed that T-MPCs were negative for CD14, CD31, CD34, and CD45 expression and positive for CD29, CD44, CD90, and CD105 expression, a characteristic phenotype of BM-MPCs. Similar to BM-MPCs, T-MPCs could be induced to undergo adipogenic differentiation and, to a lesser extent, osteogenic and chondrogenic differentiation. T-MPCs did not express class II major histocompatibility (MHC) antigens, and in a similar but less pronounced manner compared with BM-MPCs, T-MPCs were immunosuppressive, inhibiting the proliferation of T cells stimulated by allogeneic T cells or by non-specific mitogenic stimuli via an indoleamine 2,3-dioxygenase-dependent mechanism. ----- CONCLUSION: Human palatine T-MPCs represent a new source of progenitor cells, potentially applicable for cell-based therapies.
||Adolescent ; Adult ; Biopsy ; Bone Marrow Cells/cytology ; Bone Marrow Cells/drug effects ; Bone Marrow Cells/metabolism ; Cell Proliferation ; Cell Survival/physiology ; Cells, Cultured ; Child ; Child, Preschool ; Humans ; Immunosuppression ; Interferon-gamma/pharmacology ; Lymphocyte Culture Test, Mixed ; Mesenchymal Stem ; Cells/cytology ; Mesenchymal Stem Cells/drug effects ; Mesenchymal Stem Cells/metabolism ; Middle Aged ; Multipotent Stem Cells/cytology ; Multipotent Stem Cells/drug effects ; Multipotent Stem Cells/metabolism ; Palatine Tonsil/cytology ; Palatine Tonsil/drug effects ; Palatine Tonsil/metabolism ; Receptors, Interferon/metabolism
||Katedra za otorinoloringologiju s audiologijom i fonijatrijom
|Shanti, Rabie M.||UNSPECIFIED|
|Joshi, Arjun S.||UNSPECIFIED|
|Tuan, Rocky S.||UNSPECIFIED|
||23 Oct 2009
||23 Sep 2011 16:10
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