Prognostic significance of B-cell differentiation genes encoding proteins in diffuse large B-cell lymphoma and follicular lymphoma grade 3

Borovečki, Ana and Korać, Petra and Nola, Marin and Ivanković, Davor and Jakšić, Branimir and Dominis, Mara (2008) Prognostic significance of B-cell differentiation genes encoding proteins in diffuse large B-cell lymphoma and follicular lymphoma grade 3. Croatian Medical Journal, 49 (5). pp. 625-635. ISSN 0353-9504 (Print) 1332-8166 (Electronic)

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Abstract

AIM: To define prognostic significance of B-cell differentiation genes encoding proteins and BCL2 and BCL6 gene abnormalities in diffuse large B-cell lymphoma and follicular lymphoma grade 3 with >75% follicular growth pattern. ----- METHODS: In 53 patients with diffuse large B-cell lymphoma and 20 patients with follicular lymphoma grade 3 with >75% follicular growth pattern the following was performed: 1) determination of protein expression of BCL6, CD10, MUM1/IRF4, CD138, and BCL2 by immunohistochemistry; 2) subclassification into germinal center B-cell-like (GCB) and activated B-cell-like (ABC) groups according to the results of protein expression; 3) detection of t(14;18)(q32;q21)/IgH-BCL2 and BCL6 abnormalities by fluorescent in situ hybridization in diffuse large B-cell lymphoma and follicular lymphoma grade 3 with >75% follicular growth pattern as well as in GCB and ABC groups; and 4) assessment of the influence of the analyzed characteristics and clinical prognostic factors on overall survival. ----- RESULTS: Only BCL6 expression was more frequently found in follicular lymphoma grade 3 with >75% follicular growth pattern than in diffuse large B-cell lymphoma (P=0.030). There were no differences in BCL2 and BCL6 gene abnormalities between diffuse large B-cell lymphoma and follicular lymphoma grade 3 with >75% follicular growth pattern. Diffuse large B-cell lymphoma and follicular lymphoma grade 3 with >75% follicular growth pattern patients were equally distributed in GCB and ABC groups. t(14;18)(q32;q21) was more frequently recorded in GCB group, and t(14;18)(q32;q21) with BCL2 additional signals or only BCL2 and IgH additional signals in ABC group (P=0.004). The GCB and ABC groups showed no difference in BCL6 gene abnormalities. There was no overall survival difference between the diffuse large B-cell lymphoma and follicular lymphoma grade 3 with >75% follicular growth pattern patients, however, GCB group had longer overall survival than ABC group (P=0.047). Multivariate analysis showed that BCL6, CD10, and BCL2 expression, BCL2 and BCL6 abnormalities, and International Prognostic Index were not significantly related to overall survival. ----- CONCLUSION: Diffuse large B-cell lymphoma and follicular lymphoma grade 3 with >75% follicular growth pattern patients have very similar characteristics and their prognosis is more influenced by protein expression of B-cell differentiation stage genes than by tumor cells growth pattern, BCL2 and BCL6 abnormalities, and International Prognostic Index.

Item Type: Article
MeSH: DNA-Binding Proteins - analysis - genetics ; Interleukin-6 - analysis - genetics ; Lymphoma, Follicular - genetics - mortality - pathology ; Lymphoma, Large B-Cell, Diffuse - genetics - mortality - pathology ; Proto-Oncogene Proteins c-bcl-2 - analysis - genetics ; Tumor Markers, Biological - genetics ; Adult ; Aged ; Aged, 80 and over ; Female ; Gene Expression Regulation, Neoplastic ; Genetic Markers ; Humans ; Immunohistochemistry ; Male ; Middle Aged ; Neprilysin - genetics ; Predictive Value of Tests ; Prognosis ; Syndecan-1 - genetics
Departments: Katedra za internu medicinu
Katedra za patologiju
Depositing User: Boris Čičovački
Status: Published
Creators:
CreatorsEmail
Borovečki, AnaUNSPECIFIED
Korać, PetraUNSPECIFIED
Nola, MarinUNSPECIFIED
Ivanković, DavorUNSPECIFIED
Jakšić, BranimirUNSPECIFIED
Dominis, MaraUNSPECIFIED
Date: October 2008
Date Deposited: 01 Dec 2008
Last Modified: 23 Sep 2011 16:10
Subjects: /
Related URLs:
    URI: http://medlib.mef.hr/id/eprint/529

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