Distribution of hepatitis C virus genotypes in Croatia: a 10 year retrospective study of four geographic regions

Vince, Adriana and Iščić-Beš, Janja and Židovec Lepej, Snježana and Baća-Vrakela, Ivana and Bradarić, Nikola and Kurelac, Ivan and Vince, Davor B. (2006) Distribution of hepatitis C virus genotypes in Croatia: a 10 year retrospective study of four geographic regions. Collegium Antropologicum, 30 (Suppl.). pp. 139-143. ISSN 0350-6134

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Abstract

The aim of this 10-year retrospective study was to investigate the distribution of HCV genotypes in patients with chronic hepatitis C monitored in the largest center for molecular diagnostics of HCV infection in Croatia. The study enrolled 1163 anti-HCV positive adults with detectable HCV RNA in the plasma. The patients were classified in four regions: Zagreb and surrounding continental area, Split, Slavonija and Rijeka. HCV genotyping was performed by using VERSANT HCV Genotyping Assay (LIPA) (Bayer Diagnostics, Puteaux Cedex, France). Statistical analysis was performed by using Statistica for Windows V.5.1. The majority of HCV infections in the study population were caused by genotypes 1 (58.8% of infected patients) and 3 (35.6%). Percentages of patients infected with subtypes 1b and 1a were 37.4% and 13.1%, respectively. Genotypes 2 and 4 were present in a very low percentage of patients (2.2% and 3.4%, respectively) while genotypes 5 and 6 were not detected. Analysis of regional differences in the distribution of HCV genotypes revealed similar percentages of subtype 3a and 1b infections in the Split region while the majority of infections in other regions were caused by subtype 1b. Infections with genotypes 2 and 4 were present in less than 5% of patients in all geographic regions. Analysis of an association between risk factors for infection and distribution of genotypes and subtypes in a subset of patients from the Split region confirmed the association between IVDU and subtype 3a. We conclude that the prevalence of HCV genotypes and subtypes follows the pattern of other Southern and Eastern European Countries with the predominance of subtypes 1b, 3a and 1a.

Abstract in Croatian

Cilj ovog desetogodišnjeg retrospektivnog istraživanja bio je analizirati distribuciju genotipova HCV-a u bolesnika s kroničnim hepatitisom C koji su praćeni u najvećem centru za molekularnu dijagnostiku HCV infekcije u R. Hrvatskoj. U istraživanje smo uključili 1163 anti-HCV pozitivne odrasle osobe s mjerljivom HCV RNA u plazmi. Pacijenti su klasificirani u četiri regije: Zagreb i okolna kontinentalna područja, Split, Slavonija i Rijeka. Genotipovi HCV-a određivani su primjenom VERSANT HCV Genotyping Assay (LIPA) (Bayer Diagnostics, Puteaux Cedex, France). Rezultati su statistički obrađeni primjenom programa Statistica for Windows V. 5.1. Većina pacijenata uključenih u istraživanje bili su zaraženi genotipovima 1 (58,8% pacijenata) i 3 (35,6%). 37,4% pacijenata bilo je zaraženo genotipom 1b dok je infekcija genotipom 1a dokazana u 13,1% bolesnika. Broj bolesnika s genotipovima 2 i 4 (2,2% i 3,4%) bili su vrlo mali. U našoj skupini bolesnika nismo utvrdili genotipove 5 i 6. Analiza regionalne distribucije HCV genotipova pokazala je sličnu zastupljenost subtipova 3a i 1b u Splitu dok je u većine bolesnika iz drugih regija dominirala infekcija subtipom 1b. U svim analiziranim regijama genotipovi 2 i 4 bili su otkriveni u manje od 5% bolesnika. Analiza povezanosti rizičnih čimbenika za infekciju HCV-om i distribucije genotipova i subtipova u grupi bolesnika iz Splita potvrdila je povezanost između IVDU i subtipa 3a. Zaključujemo da je prevalencija genotipova i subtipova HCV-a u R. Hrvatskoj slična onoj u drugim zemljama južne i istočne Europe te da dominiraju subtipovi 1b, 3a i 1a.

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