Emergence of uropathogenic extended-spectrum beta lactamases-producing Escherichia coli strains in the community

Marijan, Tatjana and Vraneš, Jasmina and Bedenić, Branka and Mlinarić-Džepina, Ana and Plečko, Vanda and Kalenić, Smilja (2007) Emergence of uropathogenic extended-spectrum beta lactamases-producing Escherichia coli strains in the community. Collegium Antropologicum, 31 (1). pp. 227-233. ISSN 0350-6134

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Abstract

The aim of this study was to determine the virulence characteristics and resistance pattern of the extended-spectrum/lactamases (ESBLs)-producing Escherichia coli strains isolated from urine of outpatients in the Zagreb region during a five-month period, and to compare them with the non ESBLs-producing E. coli strains isolated in the same period. Out of 2451 E. coli strains isolated from urine of nonhospitalized patients with significant bacteriuria, a total of 39 ESBLs-producing strains (1.59%) were detected by a double-disk diffusion technique and by the broth-dilution minimal inhibitory concentration reduction method. The 45 non ESBLs-producing strains were randomly chosen, and phenotype of the two groups of strains was characterized and compared. Serogroup O4, hemolysin production, expression of P- and type 1 fimbriae as well as resistance to gentamicin and amikacin were significantly more prevalent characteristics among the ESBLs-producing strains than among non ESBLs-producing strains (p < 0.01), while higher prevalence of trimethoprim-sulfamethoxazole resistance among ESBLs-producing strains was not statistically significant (p > 0.05). Chromosomal DNA analysis by pulsed-field gel electrophoresis exhibited a great genomic similarity among ESBLs-producing strains and revealed that those highly virulent and resistant E. coli strains isolated from urine of outpatients in the Zagreb region had a clonal propagation.

Abstract in Croatian

Cilj ovog istraživanja bio je utvrditi virulentne značajke i rezistotip sojeva bakterije Escherichia coli koji produciraju β laktamaze proširenog spektra, izoliranih iz mokraće ambulantnih pacijenata zagrebačke regije u razdoblju od pet mjeseci, i usporediti ih sa sojevima E. coli koji ne stvaraju β laktamaze proširenog spektra, a izolirani su u istom razdoblju. Od 2451 E. coli sojeva izoliranih iz mokraće izvanbolničkih pacijenata sa signifikantnom bakteriurijom, ukupno 39 sojeva produciralo je β laktamaze proširenog spektra (1,59%), što je detektirano metodom dvostruke disk-difuzije i metodom redukcije minimalne inhibicijske koncentracije u bujonu. Slučajnim odabirom prikupljeno je 45 sojeva koji ne produciraju b laktamaze proširenog spektra, te je određen i uspoređen fenotip ove dvije grupe sojeva. Najzastupljenije značajke sojeva koji su producirali β laktamaze proširenog spektra bile su serogrupa O4, produkcija hemolizina, ispoljavanje P i tip 1 fimbrija, te rezistencija na gentamicin i amikacin (p<0,01), dok veća zastupljenost rezistencije na trimetoprim-sulfametoksazol nije bila statistički značajna (p>0,05). Kromosomska DNA analiza pomoću gel-elektroforeze u izmjeničnom polju pokazala je veliku genetsku sličnost među sojevima koji produciraju β laktamaze proširenog spektra i otkrila klonalno širenje ovih visoko virulentnih i rezistentnih uropatogenih sojeva E. coli među izvanbolničkim pacijentima zagrebačke regije.

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