Aberrant glycosylation of Igg heavy chain in multiple myeloma

Aurer, Igor and Lauc, Gordan and Dumić, Jerka and Rendić, Dubravko and Matišić, Danica and Miloš, Marija and Heffer-Lauc, Marija and Flogel, Mirna and Labar, Boris (2007) Aberrant glycosylation of Igg heavy chain in multiple myeloma. Collegium antropologicum, 31 (1). pp. 247-251. ISSN 0350-6134 (Print)

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Abstract

Although the majority of eukaryotic proteins are glycosylated, there is a dearth of knowledge regarding protein sugar moieties and their changes in disease. Most multiple myeloma cases are characterized by production of monoclonal immunoglobulins (Ig). We studied galactosylation and sialylation of IgG heavy chains in 16 patients with IgG myeloma using lectin blotting and densitometry. In comparison to age and sex matched controls, galactosylation was reduced in multiple myeloma (median 317 vs. 362, range 153-410 vs. 309-447 relative units, p = 0.015, Student's t-test). Sialylation was stage dependent; samples from patients with stage IIA had lowest amounts of sialic acid, IIIA intermediate and IIIB highest (142.6 vs. 185.9 vs. 248.5 relative units, correlation coefficient r = 0.55). Both galactosylation and sialylation levels were independent of age, sex, treatment type, response to treatment, disease duration and IgG and b2 microglobulin concentration. These data indicate that multiple myeloma is characterized by aberrant immunoglobulin glycosylation.

Abstract in Croatian

Iako je većina eukariotskih proteina glikozilirana, nema puno podataka o šećerima vezanima na proteine niti o njihovim promjenama u pojedinim bolestima. Multipli mijelom je većinom karakteriziran stvaranjem monoklonskih imunoglobulina (Ig). Istraživali smo galaktozilaciju i sijalilaciju IgG teškog lanca u 16 bolesnika s IgG mijelomom koristeći specifično vezivanje lektina i denzitometriju. U usporedbi s kontrolama podjednake dobi i spola je u multiplom mijelomu smanjena galaktozilacija (medijan 317 naprema 362, raspon 153–410 naprema 309–447 relativnih jedinica, p=0.015 koristeći Studentov t-test). Sijalilacija je ovisila o stadiju. Najmanje sijalinske kiseline je nađeno u uzorcima bolesnika sa stadijem IIA, nešto više u onih s IIIA, a najviše u IIIB stadiju (142,6 naprema 185,9 naprema 248,5 relativnih jedinica, koeficijent korelacije r=0,55). Ni razina galaktozilacije niti sijalilacije nisu ovisile o dobi, spolu, vrsti liječenja, odgovoru na liječenje, trajanju bolesti, koncentraciji IgG ili β2 mikroglobulina. Ovi podaci ukazuju da je multipli mijelom karakteriziran poremećajem glikozilacije imunoglobulina.

Item Type: Article
MeSH: Immunoglobulin G - metabolism ; Immunoglobulin Heavy Chains - metabolism ; Multiple Myeloma - immunology ; Adult ; Aged ; Female ; Galactose - metabolism ; Glycosylation ; Humans ; Male ; Middle Aged ; N-Acetylneuraminic Acid - metabolism
Departments: Katedra za internu medicinu
Katedra za medicinsku biologiju
Depositing User: Boris Čičovački
Status: Published
Creators:
CreatorsEmail
Aurer, IgorUNSPECIFIED
Lauc, GordanUNSPECIFIED
Dumić, JerkaUNSPECIFIED
Rendić, DubravkoUNSPECIFIED
Matišić, DanicaUNSPECIFIED
Miloš, MarijaUNSPECIFIED
Heffer-Lauc, MarijaUNSPECIFIED
Flogel, MirnaUNSPECIFIED
Labar, BorisUNSPECIFIED
Date: March 2007
Date Deposited: 13 Oct 2008
Last Modified: 23 Sep 2011 16:10
Subjects: /
Related URLs:
    URI: http://medlib.mef.hr/id/eprint/458

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