Tumor growth fraction, expression of estrogen and progesterone receptors, p53, bcl-2 and cathepsin D activity in primary ductal invasive breast carcinoma and their axillary lymph node metastases

Kristek, Jozo and Dmitrović, Branko and Kurbel, Sven and Šakić, Kata and Krajinović, Zlatko and Blažičević, Valerija and Has, Borislav and Marjanović, Ksenija (2007) Tumor growth fraction, expression of estrogen and progesterone receptors, p53, bcl-2 and cathepsin D activity in primary ductal invasive breast carcinoma and their axillary lymph node metastases. Collegium Antropologicum, 31 (4). pp. 1043-1047. ISSN 0350-6134

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Abstract

The aim of this paper is to determine similarities and differences between tumor cell subclones in cases of ductal invasive breast carcinoma, and which occupy primary tumor and local axillary lymph metastases. The tumor growth fraction evaluated by Ki-67 was analyzed along with the expression level of estrogen and progesterone receptors, protein p53, proto-oncogene protein bcl-2 and cathepsin D in 60 patients. Metastatic lymph node in axilla has a higher growth fraction of the tumor cells than the primary tumor (p = 0.045), as well as the higher level of bcl-2 overexpression (p = 0.014). No statistically significant difference was found in the presence of immunohistochemically identified estrogen receptors (p = 0.161) and progesterone receptors (p = 0.081) between the primary tumor and the metastatic lymph node in axilla. Likewise, no difference was found between the immunohistochemical evaluation of p53 (p = 0.356) and cathepsin D activity (p = 0.928). A higher growth fraction of the tumor cells and the higher level of bcl-2 overexpression in metastatic tumor cells indicate the more aggressive cell subclones. This study does not support the routine testing of both primary tumor and locoregional metastasis to evaluate the breast cancer hormone receptor status.

Abstract in Croatian

Cilj rada je utvrditi sličnosti i razlike između subklonova tumorskih stanica duktalnog invazivnog karcinoma dojke koji naseljavaju primarni tumor i lokalne pazušne limfne metastaze. Analizirana je diobena aktivnost tumorskih stanica izražajem Ki-67, stupanj izraženosti estrogenskih i progesteronskih biljega, proteina p53, proto-onkogena bcl-2 i aktivnost katepsina D u 60 bolesnica. Metastatski limfni čvor u pazuhu ima izraženiju diobenu aktivnost od primarnog tumora (p=0,018) i jači izražaj bcl-2 (p=0,014). Nije utvrđena statistički značajna razlika u nazočnosti imunohistokemijski određenih estrogenskih (p=0,16) i progesteronskih biljega (p=0,08) između primarnog i metastatskog tumorskog tkiva. Također nije bilo razlike između imunohistokemijske procjene p53 (p=0,356) i aktivnosti katepsina D (p=0,92). Veća diobena aktivnost i jači izražaj bcl-2 u metastatskom tkivu upućuje na agresivnije metastatske subklonove tumorskih stanica. Rezultati ne ukazuju na potrebu istovremene evaluacije hormonskih biljega u primarnom tumoru i pazušnim metastazama.

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