Expression pattern and functional analysis of mouse Stam2 in the olfactory system

Furić Čunko, Vesna and Mitrečić, Dinko and Mavrić, Sandra and Gajović, Srećko (2008) Expression pattern and functional analysis of mouse Stam2 in the olfactory system. Collegium Antropologicum, 32 (Suppl.). pp. 59-63. ISSN 0350-6134

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Abstract

Gene trap mutant mice Stam(gt1Gaj) were investigated in order to elucidate in vivo function of Stam2 (signal transducing adaptor molecule 2) gene, which was in vitro implicated in sorting cargo marked by monoubiquitination toward degradation in the lysosomes. The expression analysis showed high Stam2 expression in the brain including the regions related to olfaction, and in the olfactory epithelium, but not in the respiratory part of nasal mucosa. To test mouse olfaction, ability to find chocolate hidden under the sawdust in the cage was examined. When food was given ad libitum before trials, mutants needed more time and failed more frequently to find the chocolate. In contrast, when the mice were fasted overnight before trial, there were no differences between mutants and wild type mice. No changes in morphology of olfactory mucosa were observed. The obtained results showed the existence of phenotype differences between mutants and wild type mice. However, different results of two approaches aimed to test olfaction, with and without food deprivation, currently do not enable to assign the particular function of Stam2 to olfaction. This emphasizes how slight modification of experimental setup in behavioural testing can cause important differences on the obtained results.

Abstract in Croatian

Stam(gt1Gaj) miševi preinačeni genskom zamkom istraživani su u cilju otkrivanja uloge gena Stam2 (eng. signal transducing adaptor molecule) in vivo. Istraživanjima in vitro pokazana je uloga gena Stam2 u razvrstavanju molekula obilježenih monoubikvitinacijom prema razgradnji u lizosomima. Jak izražaj gena Stam2 u mozgu uključuje dijelove mozga koji pripadaju njušnom putu. Njušni dijelovi nosne sluznice također pokazuju jak izražaj gena Stam2, dok ga respiratorni dio ne pokazuje. Kako bi se provjerio osjet njuha miševima je ispitivana sposobnost pronalaženja čokolade skrivene ispod piljevine u kavezu. Ako je prije ispitivanja osjeta njuha hrana miševima bila dostupna ad libitum,mutantima je trebalo više vremena pronaći čokoladu i češće je nisu pronašli u zadanom vremenu. Kada su miševi bili bez hrane noć prije ispitivanja, nije bilo razlike u rezultatima između mutanata i miševa divljeg tipa. Razlike u morfologiji njušne sluznice nisu uočene. Dobiveni rezultati ukazuju na postojanje razlike u fenotipu između mutanata i miševa divljeg tipa. Različiti rezultati ispitivanja njuha u ovisnosti o dostupnosti hrane ne razjašnjavaju ima li Stam2 ulogu u osjetu njuha. Ovi podaci još jednom naglašavaju kako male promjene u postavkama ispitivanja ponašanja mogu pokazati važne razlike u konačnim rezultatima.

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