Repozitorij Medicinskog fakulteta Sveučilišta u Zagrebu

Fluid perfusion as a method of cerebrospinal fluid formation rate - critical appraisal

Orešković, Darko and Maraković, Jurica and Vukić, Miroslav and Radoš, Milan and Klarica, Marijan (2008) Fluid perfusion as a method of cerebrospinal fluid formation rate - critical appraisal. Collegium antropologicum, 32 (Suppl.). pp. 133-137. ISSN 0350-6134 (Print)

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    Croatian abstract

    Svrha prikazanih istraživanja bila je provjeriti da li perfuziona metoda izračunavanja stvaranja cerebrospinalnog likvora (Vf) po Heiseyu i sur. zaista prikazuje stvaranje likvora. U tu svrhu »Vf« je u plastičnoj komorici simuliran pomoću infuzione pumpe (40,6 mL/min), a provjera je vršena uspoređivanjem rezultata izračunanog Vf-a s pomoću Heiseyeve perfuzione metode i onog simuliranog s pomoću pumpe. U slučaju da je prefusiona metoda točna, rezultat bi trebao biti u oba slučaja isti (40,6 mL/min). U drugoj skupini pokusa vršena je ventrikulocisternalna perfuzija (brzina 92,4 mL/min) na anesteziranim i žrtvovanim mačkama. Ukoliko je ispitivana perfuziona metoda točna izračunane vrijednosti na žrtvovanim mačkama Vf-a bi morale iznositi 0 mL/min, jer ne postoji stvaranje likvora na mrtvim životinjama. Budući da se izračunani »Vf« (46,5 mL/min) u plastičnoj komorici značajno razlikuje (p<0.0001) od simuliranog s pomoću pumpe (40,6 mL/min) i budući da je izračunani Vf kod mrtvih životinja varirao od 3–5 mL/min, onda to jasno ukazuje da se perfuziona metoda ne može koristiti kao pouzdana metoda za izračunavanje stvaranja cerebrospinalnog likvora (Vf).

    English abstract

    The aim of the study was to evaluate whether or not cerebrospinal fluid formation rate (Vf) calculated according to the equation of Heisey et al., truly show the produced cerebrospinal fluid. For this reason Vf was simulated (40.6 microL/min) by an infusion pump in a plastic cylinder and the evaluation was done by comparing the results obtained between the calculated Vf and the simulated one. In both cases the result should be the same (40.6 micro/min). Other types of experiments were carried out by ventriculocisternal perfusion (92.4 microL/min) on anaesthetized and sacrificed cats. If the equation is correct, the calculated Vf for sacrificed animals should be zero, because there is no Vf in dead animals. The fact that the calculated Vf (46.5 microL/min) in the plastic cylinder was different (p < 0.0001) from the simulated one (40.6 microL/min) and that Vf was calculated even for dead animals (3-5 microL/min) clearly shows the that perfusion method may not be an accurate method for determination of Vf.

    Item Type: Article
    MeSH: Cerebral Ventricles - physiology ; Cerebrospinal Fluid - physiology ; Models, Biological ; Animals ; Cats ; Female ; Male ; Perfusion
    Divisions: Katedra za farmakologiju
    Depositing User: Boris Čičovački
    Status: Published
    Creators:
    CreatorsEmail
    Orešković, Darko
    Maraković, Jurica
    Vukić, Miroslav
    Radoš, Milan
    Klarica, Marijan
    Date: January 2008
    Date Deposited: 03 Oct 2008
    Last Modified: 23 Sep 2011 18:10
    Subjects: /
    Related URLs:
      URI: http://medlib.mef.hr/id/eprint/397

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