Quantitative analysis of basal dendritic tree of layer III pyramidal neurons in different areas of adult human frontal cortex

Zeba, Martina and Jovanov-Milošević, Nataša and Petanjek, Zdravko (2008) Quantitative analysis of basal dendritic tree of layer III pyramidal neurons in different areas of adult human frontal cortex. Collegium Antropologicum, 32 (S. 1). pp. 161-169. ISSN 0350-6134

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Abstract

Large long projecting (cortico-cortical) layer IIIc pyramidal neurons were recently disclosed to be in the basis of cognitive processing in primates. Therefore, we quantitatively examined the basal dendritic morphology of these neurons by using rapid Golgi and Golgi Cox impregnation methods among three distinct Brodmann areas (BA) of an adult human frontal cortex: the primary motor BA4 and the associative magnopyramidal BA9 from left hemisphere and the Broca's speech BA45 from both hemispheres. There was no statistically significant difference in basal dendritic length or complexity, as dendritic spine number or their density between analyzed BA's. In addition, we analyzed each of these BA's immunocytochemically for distribution of SMI-32, a marker of largest long distance projecting neurons. Within layer IIIc, the highest density of SMI-32 immunopositive pyramidal neurons was observed in associative BA9, while in primary BA4 they were sparse. Taken together, these data suggest that an increase in the complexity of cortico-cortical network within human frontal areas of different functional order may be principally based on the increase in density of large, SMI-32 immunopositive layer IIIc neurons, rather than by further increase in complexity of their dendritic tree and synaptic network.

Abstract in Croatian

Istraživanja mozga u primata ukazala su na dominantnu ulogu kortiko-kortikalnih piramidnih neurona IIIc sloja u neurobiologiji spoznajnih funkcija. U ovom radu prikazujemo rezultate usporedbene kvantitativne analize morfologije dendritičkog stabla najvećih neurona sloja IIIc impregniranih Golgi metodom, između tri različite Brodmannove areje (BA) frontalnog režnja mozga odraslog čovjeka: primarna motorna BA4, asocijativna magnopiramidalna BA9, te obostrano, govorna Brocina BA45. Statističkom analizom nismo pokazali značajne razlike umorfologiji dendritičnog stabla te broju i gustoći dendritičkih trnova između neurona različitih areja. Kvalitativna analiza laminarne distribucije i morfologije neurona označenih sa SMI-32 protutijelom, koje boji velike neurone sa dugim projekcijama, pokazala je da se u sloju III najveći broj SMI-32-imuno-reaktivnih neurona, kao i intenzitet reaktivnosti pojedinačnih neurona, nalazi u asocijativnom BA9, dok su u primarnom BA4 obojani samo poneki neuroni. Ovi podaci pokazuju da do povećanja složenosti kortiko-kortikalne mreže u funkcionalno različitim frontalnim regijama mozga dolazi prvenstveno povećanjem broja i gustoće velikih projekcijskih (kortiko-kortikalnih) neurona, a ne povećanjem njihove dendritičke i sinaptičke složenosti.

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