Serum gangliosides in patients with brain tumors

Radić, Borislav and Vukelić, Željka and Kalanj Bognar, Svjetlana (2008) Serum gangliosides in patients with brain tumors. Collegium antropologicum, 32 (Suppl.). pp. 171-175. ISSN 0350-6134 (Print)

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Abstract

In order to determine possible differences in serum gangliosides content and composition before and after surgical removal of tumor, gangliosides isolated from preoperative and postoperative sera of patients with brain tumors were analyzed. Serum samples were collected from patients with glioblastoma, meningioma, acoustic neurinoma, haemangioma, oligodendroglioma and astrocytoma, one week before and one week after surgical removal of the tumor. Serum gangliosides were qualitatively and quantitatively analyzed by high performance thin layer chromatography and laser densitometry. Results showed changes of total gangliosides concentrations in analyzed postoperative sera comparing to preoperative sera. There was not a significant difference in ganglioside pattern of preoperative vs. preoperative sera. However, a postoperative decreased proportion of ganglioside GD3 was observed in sera derived from patients with complete tumor removal. The results of this study indicate that comparative quantitative and compositional analysis of both preoperative and postoperative serum gangliosides may provide useful information concerning tumor progression, surgical success and prognosis.

Abstract in Croatian

U ovom radu analizirani su gangliozidi izolirani iz preoperativnih i postoperativnih seruma pacijenata s moždanim tumorima, kako bi se utvrdile moguće razlike u sadržaju i sastavu serumskih gangliozida prije i poslije kirurškog odstranjenja tumora. Uzorci seruma sakupljeni su od pacijenata s dijagnozom glioblastoma, meningeoma, neurinoma, hemangioma, oligodendroglioma i astrocitoma, jedan tjedan prije i jedan tjedan nakon kirurškog uklanjanja tumora. Kvalitativna i kvantitativna analiza serumskih gangliozida provedena je tankoslojnom kromatografijom visoke moći razlučivanja i laserskom denzitometrijom. Rezultati su pokazali razlike ukupnih koncentracija gangliozida u analiziranim postoperativnim serumima u usporedbi s preoperativnim serumima. Gangliozidni obrazac preoperativnih i postoperativnih seruma nije se značajno razlikovao. Međutim, uočeno je postoperativno smanjenje udjela gangliozida GD3 u serumima pacijenata u kojih je tumor potpuno uklonjen. Rezultati ovog istraživanja ukazuju da poredbena kvantitativno-kvalitativna analiza preoperativnih i postoperativnih serumskih gangliozida može pružiti korisne podatke o tumorskoj progresiji, uspješnosti kirurškog postupka i prognozi.

Item Type: Article
MeSH: Brain Neoplasms - blood - surgery ; Gangliosides - blood ; Adult ; Aged ; Chromatography, High Pressure Liquid ; Female ; Humans ; Male ; Middle Aged ; Postoperative Period ; Preoperative Care ; Prognosis
Departments: Katedra za medicinsku kemiju, biokemiju i kliničku kemiju
Hrvatski institut za istraživanje mozga
Depositing User: Boris Čičovački
Status: Published
Creators:
CreatorsEmail
Radić, BorislavUNSPECIFIED
Vukelić, ŽeljkaUNSPECIFIED
Kalanj Bognar, SvjetlanaUNSPECIFIED
Date: January 2008
Date Deposited: 03 Oct 2008
Last Modified: 23 Sep 2011 16:10
Subjects: /
Related URLs:
    URI: http://medlib.mef.hr/id/eprint/392

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