Repozitorij Medicinskog fakulteta Sveučilišta u Zagrebu

Substituting dietary polyunsaturated fat with monounsaturated fat increases insulin sensitivity in cultured rat hepatocytes

Roša, Jagoda and Baraba, Anja and Bučević, Petra and Đorđijevski, Darija and Antolković, Ivan and Roša, Josip (2008) Substituting dietary polyunsaturated fat with monounsaturated fat increases insulin sensitivity in cultured rat hepatocytes. Collegium antropologicum, 32 (1). pp. 119-123. ISSN 0350-6134 (Print)

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    English abstract

    Increased dietary fat intake generally, and saturated fat specifically, will lead to impairment of insulin action and to development of metabolic syndrome. The aim of this study was to find out changing of hepatic glucose output in dependence of high monounsaturated and high polyunsaturated fat diet and possible direct action of insulin in hepatocytes. Hepatocytes were isolated by a collagenase perfusion technique and cultured for 24 h in M 199 serum-free medium. The glucose production in hepatocytes isolated from rats on high polyunsaturated fat diet, as well as those isolated from rats on high monounsaturated fat diet was significantly higher than in standard controls. Insulin significantly decreased glucose production but only in hepatocytes obtained from rats on high monounsaturated fat diet. On the other side energy expenditure was significantly higher in rats on high monounsaturated fat diet and significantly lower in rats on high polyunsaturated fat diet comparable with animals on standard diet. Monounsaturated fat in Mediterranean diet could have beneficial effect on the development of the metabolic syndrome by increasing insulin sensitivity and energy expenditure.

    Item Type: Article
    MeSH: Insulin Resistance ; Dietary Fats, Unsaturated - pharmacology ; Hepatocytes - metabolism ; Animals ; Cells, Cultured ; Glucose - metabolism ; Male ; Rats ; Rats, Wistar ; Weight Gain
    Divisions: Katedra za fiziologiju i imunologiju
    Depositing User: Boris Čičovački
    Status: Published
    Creators:
    CreatorsEmail
    Roša, Jagoda
    Baraba, Anja
    Bučević, Petra
    Đorđijevski, Darija
    Antolković, Ivan
    Roša, Josip
    Date: March 2008
    Date Deposited: 03 Oct 2008
    Last Modified: 23 Sep 2011 18:10
    Subjects: /
    Related URLs:
      URI: http://medlib.mef.hr/id/eprint/387

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