Development of the fetal neural retina in vitro and in ectopic transplants in vivo

Katušić, Ana and Jurić-Lekić, Gordana and Jovanov-Milošević, Nataša and Vlahović, Maja and Ježek, Davor and Šerman, Ljiljana and Sinčić, Nino and Veljanovska, Biljana and Bulić-Jakuš, Floriana (2008) Development of the fetal neural retina in vitro and in ectopic transplants in vivo. Collegium antropologicum, 32 (1). pp. 201-207. ISSN 0350-6134 (Print)

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Investigation of the developmental potential of immature tissues is important for novel approaches to human regenerative medicine. Development of the fetal neural retina has therefore been investigated in two experimental systems. Retinas were microsurgically isolated from 20-days-old rat fetuses and cultivated in vitro for 12 days or transplanted in vivo under the kidney capsule of adult males for as long as 6 months. Shedding of the photoreceptor outer segment which is a process occurring at the terminal stage of photoreceptor differentiation was observed in culture by transmission electron microscopy (TEM). In transplants, no photoreceptors were found although markers of terminal neural and glial differentiation (e,g. synaptophysin, chromogranin and glial fibrilary acidic protein--GFAP) along with the molecules involved in the process of differentiation (guidance molecule semaphorin IIIA and chondroitin sulfate proteoglycan) were expressed. Semaphorin was differentially expressed being absent from older transplants. Proliferating cell nuclear antigen and nestin (marker of undifferentiated neural cells) were still weakly expressed even in six-months-old transplants. We could conclude that in both our experimental systems fetal neural retina proceeded to differentiate further on. However, even in long-term ectopic transplants a small population of cells still retained the potential for proliferation and has not yet reached the stage of terminal differentiation.

Item Type: Article
MeSH: Fetal Tissue Transplantation ; Retina - cytology - embryology - metabolism - transplantation ; Transplantation, Heterotopic ; Animals ; Cell Differentiation ; Kidney ; Male ; Rats ; Rats, Wistar ; Semaphorin-3A - metabolism
Departments: Katedra za histologiju i embriologiju
Katedra za medicinsku biologiju
Hrvatski institut za istraživanje mozga
Depositing User: Boris Čičovački
Status: Published
Jurić-Lekić, GordanaUNSPECIFIED
Jovanov-Milošević, NatašaUNSPECIFIED
Šerman, LjiljanaUNSPECIFIED
Veljanovska, BiljanaUNSPECIFIED
Bulić-Jakuš, FlorianaUNSPECIFIED
Date: March 2008
Date Deposited: 03 Oct 2008
Last Modified: 23 Sep 2011 16:10
Subjects: /
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