Repozitorij Medicinskog fakulteta Sveučilišta u Zagrebu

Development of the fetal neural retina in vitro and in ectopic transplants in vivo

Katušić, Ana and Jurić-Lekić, Gordana and Jovanov-Milošević, Nataša and Vlahović, Maja and Ježek, Davor and Šerman, Ljiljana and Sinčić, Nino and Veljanovska, Biljana and Bulić-Jakuš, Floriana (2008) Development of the fetal neural retina in vitro and in ectopic transplants in vivo. Collegium antropologicum, 32 (1). pp. 201-207. ISSN 0350-6134 (Print)

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    English abstract

    Investigation of the developmental potential of immature tissues is important for novel approaches to human regenerative medicine. Development of the fetal neural retina has therefore been investigated in two experimental systems. Retinas were microsurgically isolated from 20-days-old rat fetuses and cultivated in vitro for 12 days or transplanted in vivo under the kidney capsule of adult males for as long as 6 months. Shedding of the photoreceptor outer segment which is a process occurring at the terminal stage of photoreceptor differentiation was observed in culture by transmission electron microscopy (TEM). In transplants, no photoreceptors were found although markers of terminal neural and glial differentiation (e,g. synaptophysin, chromogranin and glial fibrilary acidic protein--GFAP) along with the molecules involved in the process of differentiation (guidance molecule semaphorin IIIA and chondroitin sulfate proteoglycan) were expressed. Semaphorin was differentially expressed being absent from older transplants. Proliferating cell nuclear antigen and nestin (marker of undifferentiated neural cells) were still weakly expressed even in six-months-old transplants. We could conclude that in both our experimental systems fetal neural retina proceeded to differentiate further on. However, even in long-term ectopic transplants a small population of cells still retained the potential for proliferation and has not yet reached the stage of terminal differentiation.

    Item Type: Article
    MeSH: Fetal Tissue Transplantation ; Retina - cytology - embryology - metabolism - transplantation ; Transplantation, Heterotopic ; Animals ; Cell Differentiation ; Kidney ; Male ; Rats ; Rats, Wistar ; Semaphorin-3A - metabolism
    Divisions: Katedra za histologiju i embriologiju
    Katedra za medicinsku biologiju
    Hrvatski institut za istraživanje mozga
    Depositing User: Boris Čičovački
    Status: Published
    Katušić, Ana
    Jurić-Lekić, Gordana
    Jovanov-Milošević, Nataša
    Vlahović, Maja
    Ježek, Davor
    Šerman, Ljiljana
    Sinčić, Nino
    Veljanovska, Biljana
    Bulić-Jakuš, Floriana
    Date: March 2008
    Date Deposited: 03 Oct 2008
    Last Modified: 23 Sep 2011 18:10
    Subjects: /
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