Serum chitotriosidase: a circulating biomarker in polycythemia vera

Krečak, Ivan and Gverić-Krečak, Velka and Rončević, Pavle and Bašić-Kinda, Sandra and Gulin, Josipa and Lapić, Ivana and Fumić, Ksenija and Ilić, Ivana and Horvat, Ivana and Zadro, Renata and Holik, Hrvoje and Coha, Božena and Peran, Nena and Aurer, Igor and Duraković, Nadira (2018) Serum chitotriosidase: a circulating biomarker in polycythemia vera. Hematology, 23 (10). pp. 793-802. ISSN 1607-8454

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Abstract

Objectives: Serum chitotriosidase activity (CHIT1) is a biomarker of macrophage activation with an important role in inflammation-induced tissue remodeling and fibrosis. Macrophages have been described to play a crucial role in regulating pathological erythropoiesis in polycythemia vera (PV). The aim of this study was to evaluate CHIT1 in patients diagnosed with Philadelphia-negative myeloproliferative neoplasms (MPNs). ----- Methods: Using fluorometric assay, we measured CHIT1 in 28 PV, 27 essential thrombocythemia (ET), 17 primary myelofibrosis (PMF), 19 patients with secondary myelofibrosis and in 25 healthy controls. ----- Results: CHIT1 was significantly higher in PV (p < .001) and post-PV myelofibrosis (MF) transformation (post-PV MF) (p = .020), but not in ET (p = .080), post-ET MF transformation (p = .086), and PMF patients (p = .287), when compared to healthy controls. CHIT1 in PV was positively correlated with hemoglobin (p = .026), hematocrit (p = .012), absolute basophil count (p = .030) and the presence of reticulin fibrosis in the bone marrow (p = .023). ----- Discussion: A positive correlation between CHIT1 and these distinct laboratory PV features might imply macrophages closely related to clonal erythropoiesis as cells of CHIT1 origin. In addition, a positive association between CHIT1 and reticulin fibrosis might indicate its potential role in PV progression. ----- Conclusion: CHIT1 might be considered as a circulating biomarker in PV. Additional studies are needed to clarify the role of CHIT1 in promoting disease progression and bone marrow fibrosis in PV.

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