Correlation between periacinar retraction clefting and the expression of E-cadherin and Beta-catenin proteins in prostatic adenocarcinoma

Limani, Rinë (2016) Correlation between periacinar retraction clefting and the expression of E-cadherin and Beta-catenin proteins in prostatic adenocarcinoma. PhD thesis, Sveučilište u Zagrebu.

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Abstract

Periacinar retraction clefting represents a criteria favoring prostatic adenocarcinoma diagnosis. In the present study, tissues from 53 cases of prostate morphologically diagnosed as prostatic adenocarcinoma and the adjacent nonneoplastic prostatic tissue were evaluated for the presence and the extent of periacinar retraction clefting. Immunohistochemistry (IHC) was used to evaluate the expression of E-cadherin and β-catenin, and this expression was compared with GSC, sPSA, positive surgical margins, BCR, TNM stage, and periacinar retraction clefting. Our study confirmed that periacinar retraction clefting is significantly more extensive in prostatic adenocarcinoma than in nonneoplastic prostatic tissue (P<0.001).We found a decreased expression of E-cadherin and β-catenin in prostatic adenocarcinoma and a negative correlation with GSC, positive surgical margins, BCR, and T stage. Periacinar clefting was positively correlated with the intensity of E-cadherin staining (rho=0.398; P=0.003), the percentage of E-cadherin staining (rho=0.367; P=0.007), the intensity of β-catenin staining (rho= 0.374; P=0.006) and the percentage of β-catenin staining (rho=0.347; P=0.011) in prostatic adenocarcinoma. However, in 4 (7.54%) samples with Gleason score (GSC) 7 (4+3) and with a mixed population of positive and negative cells, E-cadherin staining was weak to negative in the cells of tumor glands with periacinar clefts and positive in tumor glands with no periacinar clefts. This suggests that E-cadherin might play a role in the origin of periacinar clefting in higher grade tumors.

Abstract in Croatian

Periacinarne pukotine predstavljaju jedan od kriterija za dijagnozu adenokarcinoma prostate. U 53 slučajeva prostate s morfološkom dijagnozom adenokarcinoma i u okolnom neneoplastičnom tkivu prostate, određena je prisustnost i izraženost periacinarnih pukotina, i imunohistokemijska izraženost E-kadherina i ß-katenina u usporedbi s Gleason zbrojem, sPSA, pozitivnim kirurškim rubovima, biokemijskim povratkom bolesti, TNM stadijem i periacinarnim pukotinama. Naša studija potvrđuje da su periacinarne pukotine znatno jače izražene u adenokarcinomu nego u benignom tkivu prostate (p <0,001). Rezultati istraživanja pokazuju smanjenu izraženost E-kadherina i ß-katenina u adenokarcinomu prostate i negativnu korelaciju s Gleason zbrojem, pozitivnim kirurškim rubovima, biokemijskim provratkom bolesti i T stadijem. Izraženost periacinarnih pukotina je pozitivno povezana s intenzitetom ekspresije E-kadherina (p = 0.398; P = 0,003), postotkom ekspresije E-kadherina (p = 0.367; p = 0.007), intenzitetom ß-katenina (p = 0.374; p = 0,006) i postotkom ekspresije ß-katenina (rho = 0.347; P = 0.011) bojenja u adenokarcinomu prostate. U 4 (7.54%) uzoraka, Gleason zbroj 7 (4 + 3), s mješovitom populacijom pozitivnih i negativnih stanica, bojenje na E-kadherin je slabo do negativno u stanicama žlijezda s periacinarnim pukotinama i pozitivno u žlijezdama tumora bez periacinarnih pukotina. To sugerira da E-kadherin može imati ulogu u nastanku periacinarnih pukotina u tumorima prostate s većim gradusom.

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