Autologous blood coagulum is a physiological carrier for BMP6 to induce new bone formation and promote posterolateral lumbar spine fusion in rabbits

Vukičević, Slobodan and Grgurević, Lovorka and Erjavec, Igor and Pećin, Marko and Bordukalo‐Nikšić, Tatjana and Štokovic, Nikola and Lipar, Marija and Capak, Hrvoje and Matičić, Dražen and Windhager, Reinhard and Sampath, T. Kuber and Gupta, Munish (2020) Autologous blood coagulum is a physiological carrier for BMP6 to induce new bone formation and promote posterolateral lumbar spine fusion in rabbits. Journal of Tissue Engineering and Regenerative Medicine, 14 (1). pp. 147-159. ISSN 1932-6254

[img] PDF - Published Version
Download (54MB)


In the present study, we describe autologous blood coagulum (ABC) as a physiological carrier for BMP6 to induce new bone formation. Recombinant human BMP6 (rhBMP6), dispersed within ABC and formed as an autologous bone graft substitute (ABGS), was evaluated either with or without allograft bone particles (ALLO) in rat subcutaneous implants and in a posterolateral lumbar fusion (PLF) model in rabbits. ABGS induced endochondral bone differentiation in rat subcutaneous implants. Coating ALLO by ABC significantly decreased the formation of multinucleated foreign body giant cells (FBGCs) in implants, as compared with ALLO alone. However, addition of rhBMP6 to ABC/ALLO induced a robust endochondral bone formation with little or no FBGCs in the implant. In rabbit PLF model, ABGS induced new bone formation uniformly within the implant resulting in a complete fusion when placed between two lumbar transverse processes in the posterolateral gutter with an optimum dose of 100-μg rhBMP6 per ml of ABC. ABGS containing ALLO also resulted in a fusion where the ALLO was replaced by the newly formed bone via creeping substitution. Our findings demonstrate for the first time that rhBMP6, with ABC as a carrier, induced a robust bone formation with a complete spinal fusion in a rabbit PLF model. RhBMP6 was effective at low doses with ABC serving as a physiological substratum providing a permissive environment by protecting against foreign body reaction elicited by ALLO.

Item Type: Article
Additional Information: This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial‐NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made. © 2019 The Authors. Journal of Tissue Engineering and Regenerative Medicine published by John Wiley & Sons Ltd
Departments: Katedra za anatomiju i kliničku anatomiju
Depositing User: Kristina Berketa
Status: Published
Vukičević, SlobodanUNSPECIFIED
Grgurević, LovorkaUNSPECIFIED
Bordukalo‐Nikšić, TatjanaUNSPECIFIED
Štokovic, NikolaUNSPECIFIED
Matičić, DraženUNSPECIFIED
Windhager, ReinhardUNSPECIFIED
Sampath, T. KuberUNSPECIFIED
Date: January 2020
Date Deposited: 28 Aug 2020 08:26
Last Modified: 28 Aug 2020 08:26
Subjects: /
Related URLs:

Actions (login required)

View Item View Item


Downloads per month over past year