E-cadherin and beta-catenin expression patterns in malignant melanoma assessed by image analysis

Pećina-Šlaus, Nives and Žigmund, Martina and Kušec, Vesna and Nikuševa Martić, Tamara and Čačić, Mirjana and Šlaus, Mario (2007) E-cadherin and beta-catenin expression patterns in malignant melanoma assessed by image analysis. Journal of Cutaneous Pathology, 34 (3). pp. 239-246. ISSN 0303-6987 (Print) 1600-0560 (Electronic)

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BACKGROUND: We investigated the expression of E-cadherin and beta-catenin in melanoma. Both proteins are components of adherens junctions but also play signalling roles in the wnt signal transduction pathway. ----- MATERIALS AND METHODS: Seventy malignant melanomas were analysed by immunohistochemistry and evaluated by image analysis as staining density, i.e. light permeability (LP). ----- RESULTS: Comparison of mean values of relative LP for E-cadherin and beta-catenin in tumor tissue shows that levels of E-cadherin protein are significantly lower (259.67-116.23; t=22.7; p=0.000). The comparison of mean values of the relative LP of E-cadherin in melanoma to the LP in the adjacent normal skin also shows that the expression of E-cadherin in tumor is significantly lower (256.06-169.87; t=11.55, p=0.000). beta-catenin was observed in the cytoplasm in 30.6% of patients, in 24.2% in the cell membrane, in 21% in both the cytoplasm and membrane, in 1.6% in the membrane and nucleus and in 4.8% in the cytoplasm and nucleus, whereas in 17.7% of patients beta-catenin could not be observed. Patients with Clark 4 and 5 had significantly less beta-catenin than patients with Clark 2 and 3 (chi2=12.854; p=0.005). ----- CONCLUSIONS: Changes in E-cadherin and beta-catenin levels have important roles in melanoma and could be used as molecular markers of disease progression.

Item Type: Article
MeSH: Cadherins - metabolism ; Melanoma - metabolism - pathology ; Skin Neoplasms - metabolism - pathology ; beta Catenin - metabolism ; Adult ; Aged ; Aged, 80 and over ; Female ; Humans ; Image Processing, Computer-Assisted ; Immunoenzyme Techniques ; Male ; Middle Aged ; Skin - metabolism - pathology ; Tumor Markers, Biological - metabolism
Departments: Katedra za medicinsku biologiju
Katedra za sudsku medicinu
Depositing User: Lea Škorić
Status: Published
Pećina-Šlaus, NivesUNSPECIFIED
Žigmund, MartinaUNSPECIFIED
Nikuševa Martić, TamaraUNSPECIFIED
Date: March 2007
Date Deposited: 09 Apr 2008
Last Modified: 23 Sep 2011 16:10
Subjects: /
Related URLs:
    URI: http://medlib.mef.hr/id/eprint/355

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