Meningiomas exhibit loss of heterozygosity of the APC gene

Pećina-Šlaus, Nives and Nikuševa Martić, Tamara and Tomas, Davor and Beroš, Vili and Zeljko, Martina and Čupić, Hrvoje (2008) Meningiomas exhibit loss of heterozygosity of the APC gene. Journal of Neuro-Oncology, 87 (1). pp. 63-70. ISSN 0167-594X (Print) 1573-7373 (Electronic)

[img]
Preview
PDF
Download (2MB) | Preview

Abstract

The molecular mechanisms and candidate genes involved in development of meningiomas still need investigation and elucidation. In the present study 33 meningiomas were analyzed regarding genetic changes of tumor suppressor gene Adenomatous polyposis coli (APC), a component of the wnt signaling. Gene instability was tested by polymerase chain reaction/loss of heterozygosity (LOH) using Restriction Fragment Length Polymorphism (RFLP) method. RFLP was performed by two genetic markers, Rsa I in APC's exon 11 and Msp I in its exon 15. The results of our analysis showed altogether 15 samples with LOH of the APC gene out of 32 heterozygous patients (47%). Seven patients had LOHs at both exons, while four LOHs were exclusive for exon 11 and four for exon 15. The changes were distributed according to pathohistological grade as follows: 46% of meningothelial meningioma showed LOH; 33% of fibrous; 75% of mixed (transitional); 75% of angiomatous, and one LOH was found in a single case of psammomatous meningioma. None of the LOHs were found in atypical and anaplastic cases. Immunostaining showed that samples with LOHs were accompanied with the absence of APC protein expression or presence of mutant APC proteins (chi(2 )= 13.81, df = 2, P < 0.001). We also showed that nuclear localization of beta-catenin correlates to APC genetic changes (chi(2 )= 21.96, df = 2, P < 0.0001). The results of this investigation suggest that genetic changes of APC gene play a role in meningioma formation.

Item Type: Article
MeSH: Genes, APC ; Loss of Heterozygosity ; Meningeal Neoplasms - genetics - pathology ; Meningioma - genetics - pathology ; Adult ; Aged ; Female ; Humans ; Immunohistochemistry ; Male ; Middle Aged ; Polymorphism, Restriction Fragment Length
Departments: Katedra za medicinsku biologiju
Depositing User: Lea Škorić
Status: Published
Creators:
CreatorsEmail
Pećina-Šlaus, NivesUNSPECIFIED
Nikuševa Martić, TamaraUNSPECIFIED
Tomas, DavorUNSPECIFIED
Beroš, ViliUNSPECIFIED
Zeljko, MartinaUNSPECIFIED
Čupić, HrvojeUNSPECIFIED
Date: March 2008
Date Deposited: 01 Apr 2008
Last Modified: 23 Sep 2011 16:10
Subjects: /
Related URLs:
    URI: http://medlib.mef.hr/id/eprint/341

    Actions (login required)

    View Item View Item

    Downloads

    Downloads per month over past year