Radulović, Petra and Krušlin, Božo (2018) Immunohistochemical expression of NEDD9, E-cadherin and γ-catenin and their prognostic significance in pancreatic ductal adenocarcinoma (PDAC). Bosnian Journal of Basic Medical Sciences, 18 (3). pp. 246-251. ISSN 1840-4812
![[img]](http://medlib.mef.hr/style/images/fileicons/application_pdf.png) |
PDF
Download (1MB) |
Abstract
Extensive research is being conducted to identify novel diagnostic, predictive and prognostic biomarkers for pancreatic ductal adenocarcinoma (PDAC), as only a few markers have been routinely used so far with limited success. Our aim was to assess the expression of neural precursor cell expressed developmentally down-regulated protein 9 (NEDD9), E-cadherin, and γ-catenin in PDAC in relation to clinicopathological parameters and patient survival. We also investigated if there is a correlation of NEDD9 expression with E-cadherin or γ-catenin. The protein expression was determined by immunohistochemistry in 61 PDAC and 61 samples of normal pancreatic tissue. The log rank test and Kaplan- Meier survival curve were used for survival analysis. E-cadherin and γ-catenin expressions were reduced in PDAC, and completely retained in normal pancreatic tissue. Expression of NEDD9 was significantly increased in PDAC (strong expression in 78.7% of cases and moderate in 21.3%) and reduced in normal pancreatic tissue (strong positivity in 45.9% of cases, moderate in 31.1%, and weak in 23%). There was a positive correlation between reduced E-cadherin and γ-catenin expression in PDAC (p = 0.015). The loss or reduced expression of E-cadherin had a negative impact on patient survival (p = 0.020). A negative correlation between E-cadherin expression and tumor grade was also observed (p = 0.011). Decreased E-cadherin expression was more common in male patients with PDAC (81.3% vs. 60% for females, p = 0.005). γ-catenin and NEDD9 expressions were not statistically correlated with tumor stage and grade, gender, nor with patient survival. Our results support the role of NEDD9, E-cadherin and γ-catenin proteins in PDAC, but further research should clarify in detail their mechanism of action in pancreatic cancer.
| Item Type: | Article | ||||||
|---|---|---|---|---|---|---|---|
| Additional Information: | The Bosnian Journal of Basic Medical Sciences (BJBMS) is open access, international, peer-reviewed journal, publishing articles in different disciplines of basic medical sciences. BJBMS welcomes original research and comprehensive reviews as well as short research communications in the field of molecular biology, biochemistry, genetics, immunology, microbiology, pathology, pharmacology, pharmaceutical sciences, physiology, and translational research. | ||||||
| MeSH: | Adaptor Proteins, Signal Transducing/metabolism ; Adult ; Aged ; Antigens, CD/metabolism ; Cadherins/metabolism ; Carcinoma, Pancreatic Ductal/diagnosis ; Carcinoma, Pancreatic Ductal/metabolism ; Female ; Follow-Up Studies ; Gene Expression Regulation, Neoplastic ; Humans ; Immunohistochemistry ; Kaplan-Meier Estimate ; Male ; Middle Aged ; Pancreatic Neoplasms/diagnosis ; Pancreatic Neoplasms/metabolism ; Phosphoproteins/metabolism ; Prognosis ; gamma Catenin/metabolism | ||||||
| Departments: | Katedra za patologiju | ||||||
| Depositing User: | Kristina Berketa | ||||||
| Status: | Published | ||||||
| Creators: |
|
||||||
| Date: | August 2018 | ||||||
| Date Deposited: | 19 Jul 2019 09:54 | ||||||
| Last Modified: | 19 Jul 2019 09:54 | ||||||
| Subjects: | / | ||||||
| Related URLs: | |||||||
| URI: | http://medlib.mef.hr/id/eprint/3349 |
Actions (login required)
 |
View Item |
Downloads
Downloads per month over past year
