Influence of hyperthermal regimes on experimental teratoma development in vitro

Katušić Bojanac, Ana and Rogošić, Srđan and Sinčić, Nino and Jurić-Lekić, Gordana and Vlahovic, Maja and Šerman, Ljiljana and Ježek, Davor and Bulić-Jakuš, Floriana (2018) Influence of hyperthermal regimes on experimental teratoma development in vitro. International Journal of Experimental Pathology, 99 (3). pp. 131-144. ISSN 1365-2613

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We screened for the impact of hyperthermal regimes varying in the cumulative equivalent minutes at 43°C (CEM43°C) and media composition on tumour development using an original teratoma in vitro model. Rat embryos (three germ layers) were microsurgically isolated and cultivated at the air-liquid interface. During a two week period, ectodermal, mesodermal and endodermal derivatives developed within trilaminar teratomas. Controls were grown at 37°C. Overall growth was measured, and teratoma survival and differentiation were histologically assessed. Cell proliferation was stereologically quantified by the volume density of Proliferating Cell Nuclear Antigen. Hyperthermia of 42°C, applied for 15 minutes after plating (CEM43°C 3.75 minutes), diminished cell proliferation (P ˂ .0001) and enhanced differentiation of both myotubes (P ˂ .01) and cylindrical epithelium (P ˂ .05). Hyperthermia of 43°C applied each day for 30 minutes during the first week (CEM43°C 210 minutes) impaired overall growth (P ˂ .01) and diminished cell proliferation (P ˂ .0001). Long-term hyperthermia of 40.5°C applied for two weeks (CEM43°C 630 minutes) significantly impaired survival (P ˂ .005). Long-term hyperthermia of 40.5°C applied from the second day when differentiation of tissues begins (CEM43°C 585 minutes) impaired survival (P ˂ .0001), overall growth (P ˂ .01) and cartilage differentiation (P ˂ .05). No teratomas survived extreme regimes: 43°C for 24 hours (CEM43°C 1440 minutes), hyperthermia in the scant serum-free medium (CEM43°C 630 minutes) or treatment with an anti-HSP70 antibody before long-term hyperthermia 40.5°C from the second day (CEM43°C 585 minutes). This in vitro research provided novel insights into the impact of hyperthermia on the development of experimental teratomas from their undifferentiated sources and are thus of potential interest for future therapeutic strategies in corresponding in vivo models.

Item Type: Article
Additional Information: This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. © 2018 The Authors. International Journal of Experimental Pathology published by John Wiley & Sons Ltd on behalf of Company of the International Journal of Experimental Pathology (CIJEP).
MeSH: Animals ; Cell Differentiation ; Cell Proliferation ; Cell Survival ; Embryo Culture Techniques ; Embryo, Mammalian/metabolism ; Embryo, Mammalian/pathology ; Female ; Hyperthermia, Induced/methods ; Pregnancy ; Proliferating Cell Nuclear Antigen/metabolism ; Rats, Inbred F344 ; Teratoma/metabolism ; Teratoma/pathology ; Teratoma/prevention & control ; Time Factors
Departments: Katedra za histologiju i embriologiju
Katedra za medicinsku biologiju
Depositing User: Kristina Berketa
Status: Published
Katušić Bojanac, AnaUNSPECIFIED
Jurić-Lekić, GordanaUNSPECIFIED
Šerman, LjiljanaUNSPECIFIED
Bulić-Jakuš, FlorianaUNSPECIFIED
Date: June 2018
Date Deposited: 19 Jul 2019 09:37
Last Modified: 19 Jul 2019 09:37
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