Podrimaj-Bytyqi, Arjeta and Borovečki, Ana and Selimi, Qerim and Manxhuka-Kerliu, Suzana and Gashi, Goneta and Elezaj, Isa R. (2018) The frequencies of micronuclei, nucleoplasmic bridges and nuclear buds as biomarkers of genomic instability in patients with urothelial cell carcinoma. Scientific Reports, 8 (1). p. 17873. ISSN 2045-2322
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Abstract
Bladder urothelial cell carcinoma (UCC) is an increasingly prevalent cancer worldwide, and thus, gaining a better understanding of its identifiable risk factors is a global priority. This study addressed this public health need with the understanding that cancer-initiating events, such as chromosome breakage, loss and rearrangement, can be reasonably used as biomarkers to evaluate an individual's cancer risk. Overall, forty bladder cancer patients and twenty controls were evaluated for genomic instability. To the best of the investigators' knowledge, this is the first study to perform micronucleus (MN) assays simultaneously in urothelial exfoliated cells (UEC), buccal exfoliated cells (BEC), and peripheral blood lymphocytes (PBL) in first-diagnosed, non-smoker bladder UCC patients. Additionally, the frequency of nucleoplasmic bridges (NPBs) and nuclear buds (NBUDs) in PBL was evaluated. The MN frequencies in UEC, BEC, and PBL, as well as the frequencies of NPBs and NBUDs, were significantly higher in patients than in controls. In conclusion, MN assays, particularly in UEC, may be used to identify individuals who are at high risk of developing UCC, as single or as additional triage test to UroVysion FISH test. Our results further validate the efficacy of biomarkers, such as MN, NPBs, and NBUDs, as predictors of genomic instability.
| Item Type: | Article | ||||||||||||||
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| Additional Information: | Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. © The Author(s) 2018 | ||||||||||||||
| Departments: | Katedra za socijalnu medicinu i organizaciju zdravstvene zaštite | ||||||||||||||
| Depositing User: | Anja Majstorović | ||||||||||||||
| Status: | Published | ||||||||||||||
| Creators: |
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| Date: | 14 December 2018 | ||||||||||||||
| Date Deposited: | 26 Jun 2019 07:35 | ||||||||||||||
| Last Modified: | 26 Jun 2019 07:35 | ||||||||||||||
| Subjects: | / | ||||||||||||||
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| URI: | http://medlib.mef.hr/id/eprint/3307 |
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