Counteraction of perforated cecum lesions in rats: effects of pentadecapeptide BPC 157, L-NAME and L-arginine

Drmić, Domagoj and Samara, Mariam and Vidović, Tinka and Malekinušić, Dominik and Antunović, Marko and Vrdoljak, Borna and Ruzman, Jelena and Milković Periša, Marija and Horvat Pavlov, Katarina and Jeyakumar, Jerusha and Seiwerth, Sven and Sikirić, Predrag (2018) Counteraction of perforated cecum lesions in rats: effects of pentadecapeptide BPC 157, L-NAME and L-arginine. World Journal of Gastroenterology, 24 (48). pp. 5462-5476. ISSN 1007-9327

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Abstract

AIM: To study the counteraction of perforated cecum lesion using BPC 157 and nitric oxide (NO) system agents. ----- METHODS: Alongside with the agents' application (after 1 min, medication (/kg, 10 mL/2 min bath/rat) includes: BPC 157 (10 μg), L-NAME (5 mg), L-arginine (100mg) alone or combined, and saline baths (controls)) on the rat perforate cecum injury, we continuously assessed the gross reappearance of the vessels (USB microcamera) quickly propagating toward the defect at the cecum surface, defect contraction, bleeding attenuation, MDA- and NO-levels in cecum tissue at 15 min, and severity of cecum lesions and adhesions at 1 and 7 d. ----- RESULTS: Post-injury, during/after a saline bath, the number of vessels was significantly reduced, the defect was slightly narrowed, bleeding was significant and MDA-levels increased and NO-levels decreased. BPC 157 bath: the vessel presentation was markedly increased, the defect was noticeably narrowed, the bleeding time was shortened and MDA- and NO-levels remained normal. L-NAME: reduced vessel presentation but not more than the control, did not change defect and shortened bleeding. L-arginine: exhibited less vessel reduction, did not change the defect and prolonged bleeding. In combination, mutual counteraction occurred (L-NAME + L-arginine) or the presentation was similar to that of BPC 157 rats (BPC 157 + L-NAME; BPC 157 + L-arginine; BPC 157 + L-NAME + L-arginine), except the defect did not change. Thereby at day 1 and 7, saline, L-NAME, L-arginine and L-NAME + L-arginine failed (defect was still open and large adhesions present). ----- CONCLUSION: The therapeutic effect was achieved with BPC 157 alone or in combination with L-NAME and L-arginine as it was able to consolidate the stimulating and inhibiting effects of the NO-system towards more effective healing recruiting vessels.

Item Type: Article
Additional Information: This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/ licenses/by-nc/4.0/
MeSH: Animals ; Anti-Ulcer Agents/pharmacology ; Anti-Ulcer Agents/therapeutic use ; Arginine/pharmacology ; Arginine/therapeutic use ; Cecum/blood supply ; Cecum/drug effects ; Cecum/injuries ; Disease Models, Animal ; Drug Therapy, Combination/methods ; Humans ; Intestinal Perforation/drug therapy ; Male ; NG-Nitroarginine Methyl Ester/pharmacology ; NG-Nitroarginine Methyl Ester/therapeutic use ; Nitric Oxide/metabolism ; Peptide Fragments/pharmacology ; Peptide Fragments/therapeutic use ; Proteins/pharmacology ; Proteins/therapeutic use ; Rats ; Rats, Wistar ; Treatment Outcome ; Wound Healing/drug effects
Departments: Katedra za farmakologiju
Katedra za patologiju
Depositing User: Anja Majstorović
Status: Published
Creators:
CreatorsEmail
Drmić, DomagojUNSPECIFIED
Samara, MariamUNSPECIFIED
Vidović, TinkaUNSPECIFIED
Malekinušić, DominikUNSPECIFIED
Antunović, MarkoUNSPECIFIED
Vrdoljak, BornaUNSPECIFIED
Ruzman, JelenaUNSPECIFIED
Milković Periša, MarijaUNSPECIFIED
Horvat Pavlov, KatarinaUNSPECIFIED
Jeyakumar, JerushaUNSPECIFIED
Seiwerth, SvenUNSPECIFIED
Sikirić, PredragUNSPECIFIED
Date: 28 December 2018
Date Deposited: 19 Jun 2019 10:02
Last Modified: 25 Aug 2020 07:27
Subjects: /
Related URLs:
URI: http://medlib.mef.hr/id/eprint/3286

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