Repozitorij Medicinskog fakulteta Sveučilišta u Zagrebu

Oxidative stress parameters in plasma of Huntington's disease patients, asymptomatic Huntington's disease gene carriers and healthy subjects : A cross-sectional study

Klepac, Nataša and Relja, Maja and Klepac, Ratimir and Hećimović, Silva and Babić, Tomislav and Trkulja, Vladimir (2007) Oxidative stress parameters in plasma of Huntington's disease patients, asymptomatic Huntington's disease gene carriers and healthy subjects : A cross-sectional study. Journal of Neurology, 254 (12). pp. 1676-1683. ISSN 0340-5354 (Print) 1432-1459 (Electronic)

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    English abstract

    BACKGROUND : Animal data and postmortem studies suggest a role of oxidative stress in the Huntington's disease (HD), but in vivo human studies have been scarce. ----- AIM : To assess the presence of oxidative stress in HD patients and its occurrence relative to clinical symptoms. ----- METHODS : Oxidative stress markers were determined in plasma of HD patients (n = 19), asymptomatic HD gene carriers (with > 38 CAG repeats) (n = 11) and their respective sex and agematched healthy controls (n = 47 and n = 22) in a cross-sectional study. ----- RESULTS : With adjustment for age and sex, HD patients had higher plasma lipid peroxidation (LP) levels (ratio 1.20, 95% CI 1.09 to 1.32, p < 0.001) and lower reduced glutathione (GSH) levels (ratio 0.72, CI 0.55 to 0.94, p = 0.011) than their age and sex-matched controls. Although considerably younger, HD gene carriers did not differ from HD patients regarding LP and GSH levels, and had higher plasma LP (ratio 1.16, CI 1.02 to 1.32, p = 0.016) and lower GSH than their matched controls (ratio 0.73, CI 0.5 to 1.05). They had higher LP (ratio 1.18, CI 1.02 to 1.34, p = 0.019) and lower GSH (ratio 0.75, CI 0.51 to 1.11) than the healthy subjects matched to HD patients. ----- CONCLUSIONS : Oxidative stress is more pronounced in HD patients and asymptomatic HD gene carriers than in healthy subjects. Differences in plasma LP and GSH are in line with the brain findings in animal models of HD. Data suggest that oxidative stress occurs before the onset of the HD symptoms.

    Item Type: Article
    MeSH: Huntington Disease - blood - genetics - physiopathology ; Lipids - blood ; Oxidative Stress - genetics - physiology ; Plasma - metabolism ; Adult ; Case-Control Studies ; Cross-Sectional Studies ; Female ; Glutathione Peroxidase - blood ; Humans ; Lipid Peroxidation - genetics ; Male ; Middle Aged ; Statistics, Nonparametric ; Trinucleotide Repeat Expansion - genetics ; Verbal Behavior - physiology
    Divisions: Katedra za farmakologiju
    Katedra za medicinsku biologiju
    Katedra za neurologiju
    Depositing User: Lea Škorić
    Status: Published
    Creators:
    CreatorsEmail
    Klepac, Nataša
    Relja, Maja
    Klepac, Ratimir
    Hećimović, Silva
    Babić, Tomislav
    Trkulja, Vladimir
    Date: December 2007
    Date Deposited: 25 Jan 2008
    Last Modified: 23 Sep 2011 18:10
    Subjects: /
    Related URLs:
      URI: http://medlib.mef.hr/id/eprint/324

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