Ekspresija plakofilina 3 u difuznom malignom pleuralnom mezoteliomu [Expression of plakophilin 3 in diffuse malignant pleural mesothelioma]

Mašić, Silvija (2018) Ekspresija plakofilina 3 u difuznom malignom pleuralnom mezoteliomu [Expression of plakophilin 3 in diffuse malignant pleural mesothelioma]. PhD thesis, Sveučilište u Zagrebu.

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Abstract

Diffuse malignant pleural mesothelioma (DMPM) is the most common primary malignant pleural neoplasm which implies diagnostic, prognostic and therapeutic challenges. Plakophilin 3 is a protein incorporated in desmosomes of stratified and simple epithelia of healthy tissues, but also malignant cells of various tumors where it contributes to process of tumorigenesis. This study aimed to investigate expression of plakophilin 3 in DMPM, its prognostic significance and connection to histological parameters of tumor spread. Used were 100 archival samples of DMPM tissue and classified into three histological types of DMPM (epithelioid, sarcomatoid, biphasic). Additional subclassification of epithelioid mesotheliomas based on the prevalent histological component of the tumor was performed. After immunohistochemical staining, cytoplasmic and membranous tumor cells immunopositivity was assesed by scoring the intensity of staining from 0 ( no staining) to 4 (very strong staining). Prognostic value and expression of plakophilin 3 with consideration to histologically estimated tumor spread were statistically analyzed using non- parametric tests. The results demonstrated higher level of plakophilin 3 expression in tumor samples with histologically estimated spread to fatty tissue and/ or skeletal muscle. Prognostic value was not statistically significant. This research suggests plakophilin 3 to be involved in tumor spread of DMPM.

Abstract in Croatian

Difuzni maligni pleuralni mezoteliom (DMPM) je najčešća primarna maligna neoplazma pleure i predstavlja dijagnostički, prognostički i terapeutski izazov. Plakofilin 3 je protein te je sastavni dio dezmosoma složenog i jednostavnog epitela zdravih tkiva, ali i malignih stanica različitih tumora te pridonosi procesu tumorigeneze. Cilj ovog istraživanja je istražiti izraženost plakofilina 3 u DMPM- u, njegovu prognostičku vrijednost i povezanost s histološki procijenjenim širenjem tumora. Korišteno je 100 arhivskih uzoraka DMPM-a, klasificirano u 3 glavna tipa DMPM-a (epiteloidni, sarkomatoidni, bifazični) i podtipove epiteloidnog mezotelioma na temelju prevladavajuće histološke komponente. Nakon imunohistokemijskog bojenja, analiziran je intenzitet bojenja plakofilina 3 u membrani i citoplazmi te bodovan od 0 (nema bojenja) do 4 ( izrazito jako bojenje). Upotrebom neparametrijskih testova analizirana je prognostička vrijednost i izraženost plakofilina 3 obzirom na histološki procijenjeno širenje tumora. Rezultati pokazuju da je plakofilin 3 bio bolje izražen u uzorcima s histološki procijenjenim širenjem tumora u masno tkivo i/ili skeletni mišić, u odnosu na uzorke sa širenjem u vezivo, dok prognostički značaj nije pokazan. Na temelju ovog istraživanja možemo zaključiti da plakofilin 3 koordinira u procesu širenja DMPM-a.

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