Impact of surface functionalization on the uptake mechanism and toxicity effects of silver nanoparticles in HepG2 cells

Brkić Ahmed, Lada and Milić, Mirta and Pongrac, Igor M. and Marjanović, Ana Marija and Mlinarić, Hrvoje and Pavičić, Ivan and Gajović, Srećko and Vinković Vrček, Ivana (2017) Impact of surface functionalization on the uptake mechanism and toxicity effects of silver nanoparticles in HepG2 cells. Food and Chemical Toxicology, 107 (Pt. A). pp. 349-361. ISSN 0278-6915

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Abstract

Safe and successful bioapplications of metallic nanoparticles depend on their physicochemical characteristics, in particular their surface properties. This study aimed to investigate how different surface functionalization of silver nanoparticles (AgNP) affect their interaction with mammalian liver cells with regard to cytotoxicity, genotoxicity and mechanism of cellular uptake. Differentially coated AgNP were prepared by surface functionalization using sodium bis(2-ethylhexyl)-sulfosuccinate (AOTAgNP), cetyltrimethylammonium bromide (CTABAgNP), poly(vinylpyrrolidone) (PVPAgNP), poly-l-lysine (PLLAgNP), and bovine serum albumin (BSAAgNP). Data showed varying toxic potential of differentially coated AgNP. All AgNP types demonstrated concentration dependent effects on cytotoxicity and genotoxicity in HepG2 cells. Cytotoxic potential of differentially coated AgNP followed the order of BSAAgNP > PLLAgNP > CTABAgNP > AOTAgNP > PVPAgNP. Exposure of HepG2 cells to non-cytotoxic concentrations (up to 10 mg Ag/L) of AgNP for 24 h induced primary DNA damage as evaluated by alkaline comet assay. The highest increase in both comet tail length and tail intensity was produced by PLLAgNP followed by AOTAgNP, while CTABAgNP appeared to be least damaging. The main uptake mechanisms of AgNP were macropinocytosis and clathrin-mediated endocytosis. The study findings contribute to the criteria that should be considered in evaluating the biocompatibility and safety of novel nanomaterials.

Item Type: Article
MeSH: Comet Assay ; DNA Damage/drug effects ; Endocytosis ; Hep G2 Cells ; Humans ; Metal Nanoparticles/chemistry ; Metal Nanoparticles/toxicity ; Particle Size ; Silver/chemistry ; Silver/metabolism ; Silver/toxicity ; Surface Properties
Departments: Hrvatski institut za istraživanje mozga
Katedra za histologiju i embriologiju
Depositing User: Martina Žužak
Status: Published
Creators:
CreatorsEmail
Brkić Ahmed, LadaUNSPECIFIED
Milić, MirtaUNSPECIFIED
Pongrac, Igor M.UNSPECIFIED
Marjanović, Ana MarijaUNSPECIFIED
Mlinarić, HrvojeUNSPECIFIED
Pavičić, IvanUNSPECIFIED
Gajović, SrećkoUNSPECIFIED
Vinković Vrček, IvanaUNSPECIFIED
Date: September 2017
Date Deposited: 23 Sep 2019 07:25
Last Modified: 17 Aug 2020 08:47
Subjects: /
Related URLs:
URI: http://medlib.mef.hr/id/eprint/2861

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