Cervical HPV type-specific pre-vaccination prevalence and age distribution in Croatia.

Sabol, Ivan and Milutin Gašperov, Nina and Matovina, Mihaela and Božinović, Ksenija and Grubišić, Goran and Fistonić, Ivan and Belci, Dragan and Alemany, Laia and Džebro, Sonja and Dominis, Mara and Šekerija, Mario and Tous, Sara and de Sanjosé, Silvia and Grce, Magdalena (2017) Cervical HPV type-specific pre-vaccination prevalence and age distribution in Croatia. PLoS ONE, 12 (7). e0180480. ISSN 1932-6203

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The main etiological factor of precancerous lesion and invasive cervical cancer are oncogenic human papillomaviruses types (HPVs). The objective of this study was to establish the distribution of the most common HPVs in different cervical lesions and cancer prior to the implementation of organized population-based cervical screening and HPV vaccination in Croatia. In this study, 4,432 cervical specimens, collected through a 16-year period, were tested for the presence of HPV-DNA by polymerase chain reaction (PCR) with three sets of broad-spectrum primers and type-specific primers for most common low-risk (LR) types (HPV-6, 11) and the most common high-risk (HR) types (HPV-16, 18, 31, 33, 45, 52, 58). Additional 35 archival formalin-fixed, paraffin embedded tissue of cervical cancer specimens were analyzed using LiPA25 assay. The highest age-specific HPV-prevalence was in the group 18-24 years, which decreased continuously with age (P<0.0001) regardless of the cytological diagnosis. The prevalence of HR-HPV types significantly increased (P<0.0001) with the severity of cervical lesions. HPV-16 was the most common type found with a prevalence (with or without another HPV-type) of 6.9% in normal cytology, 15.5% in atypical squamous cells of undetermined significance, 14.4% in low-grade squamous intraepithelial lesions, 33.3% in high-grade squamous intraepithelial lesions, and 60.9% in cervical cancer specimens (P<0.0001). This study provides comprehensive and extensive data on the distribution of the most common HPV types among Croatian women, which will enable to predict and to monitor the impact of HPV-vaccination and to design effective screening strategies in Croatia.

Item Type: Article
Additional Information: © 2017 Sabol et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
MeSH: Adolescent ; Adult ; Age Distribution ; Aged ; Aged, 80 and over ; Croatia/epidemiology ; Female ; Humans ; Incidence ; Middle Aged ; Papillomaviridae/classification ; Papillomaviridae/immunology ; Papillomavirus Infections/epidemiology ; Papillomavirus Infections/immunology ; Papillomavirus Infections/virology ; Prevalence ; Uterine Cervical Neoplasms/immunology ; Uterine Cervical Neoplasms/mortality ; Uterine Cervical Neoplasms/virology ; Vaccination ; Young Adult
Departments: Katedra za patologiju
Depositing User: Martina Žužak
Status: Published
Milutin Gašperov, NinaUNSPECIFIED
Matovina, MihaelaUNSPECIFIED
Božinović, KsenijaUNSPECIFIED
Grubišić, GoranUNSPECIFIED
Šekerija, MarioUNSPECIFIED
de Sanjosé, SilviaUNSPECIFIED
Grce, MagdalenaUNSPECIFIED
Date: 10 July 2017
Date Deposited: 14 Mar 2018 10:54
Last Modified: 14 Mar 2018 10:54
Related URLs:
URI: http://medlib.mef.hr/id/eprint/2850

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