The comparison of Th1, Th2, Th9, Th17 and Th22 cytokine profiles in acute and chronic HIV-1 infection.

Gorenec, Lana and Židovec Lepej, Snježana and Grgić, Ivana and Planinić, Ana and Iščić Beš, Janja and Vince, Adriana and Begovac, Josip (2016) The comparison of Th1, Th2, Th9, Th17 and Th22 cytokine profiles in acute and chronic HIV-1 infection. Microbial Pathogenesis, 97. pp. 125-30. ISSN 0882-4010

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Abstract

The aim of this study was to compare cytokine expression on both gene and protein levels in acute and chronic phase of HIV type 1 (HIV-1) infection. Thirty four patients were enrolled for cytokine expression analysis on protein level in acute and chronic stage of HIV-1 infection. Using PCR array technology, expression of 84 cytokine genes was measured in 3 patients in acute and 3 patients in chronic stage of HIV-1 infection. Bead-based cytometry was used to quantify levels of Th1/Th2/Th9/Th17/Th22 cytokines. The results showed statistically significant increase of 13 cytokine gene expression (cd40lg, csf2, ifna5, il12b, il1b, il20, lta, osm, spp1, tgfa, tnfsf 11, 14 and 8) and downregulation of the il12a expression in chronic HIV type 1 infection. Concentrations of IL-10, IL-4 and TNF-α were increased in the acute HIV type 1 infection when compared to control group. During chronic HIV type 1 infection there was an increase of IL-10, TNF-α, IL-2, IL-6, IL-13 and IL-22 levels when compared to control group. Comparison of cytokine expression between two stages of infection showed a significant decrease in IL-9 concentration. This study showed changes in cytokine profiles on both gene and protein levels in different stages of HIV-infection.

Item Type: Article
MeSH: Croatia ; Cytokines/analysis ; Cytokines/genetics ; Flow Cytometry ; Gene Expression Profiling ; HIV Infections/immunology ; HIV Infections/virology ; HIV-1/immunology ; Hospitals, University ; Humans ; Microarray Analysis ; Polymerase Chain Reaction ; Retrospective Studies ; T-Lymphocytes, Helper-Inducer/immunology
Departments: Katedra za infektologiju
Depositing User: Martina Žužak
Status: Published
Creators:
CreatorsEmail
Gorenec, LanaUNSPECIFIED
Židovec Lepej, SnježanaUNSPECIFIED
Grgić, IvanaUNSPECIFIED
Planinić, AnaUNSPECIFIED
Iščić Beš, JanjaUNSPECIFIED
Vince, AdrianaUNSPECIFIED
Begovac, JosipUNSPECIFIED
Date: August 2016
Date Deposited: 10 Nov 2017 10:21
Last Modified: 10 Nov 2017 10:21
Subjects: UNSPECIFIED
Related URLs:
URI: http://medlib.mef.hr/id/eprint/2745

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