Circulating dipeptidyl peptidase-4 activity is associated with insulin resistance in type 1 diabetic patients

Blaslov, Kristina and Bulum, Tomislav and Duvnjak, Lea (2015) Circulating dipeptidyl peptidase-4 activity is associated with insulin resistance in type 1 diabetic patients. Journal of Diabetes and its Complications, 29 (3). pp. 390-394. ISSN 1056-8727

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Abstract

AIM: The pathophysiology of insulin resistance (IR) comprises a complex adipokine mediated cross-talk between white adipose tissue and other organs. Dipeptidyl peptidase-4 (DPP4) is protease recently proposed as a novel adipokine linked to IR. We aimed to assess the relationship between fasting serum DPP4 activityand IR in type 1 diabetic (T1DM) patients. ----- METHODS: A cross-sectional study comprised 44 T1DM patients aged >18 and <65years. IR was esimated using the equation for insulin sensitivity derived from euglycemic-hyperinsulinemic clamp studies-estimated glucose disposal rate (eGDR). DPP4 serum activity was determined spectrophotometrically as a rate of cleavage of 7-Amino-4-Methyl Coumarin (AMC) from H-Gly-Pro-AMC. ----- RESULTS: Patients were divided according to DPP4 activity tertiles (<25.40; ≥36.54 U/L). Fasting serum DPP4 activity was related to disease duration (p=0.012), systolic (p=0.009) and diastolic (p=0.047) blood pressure, waist circumference (p=0.037), urine albumin excretion (p=0.022) and conversely related to eGDR (p=0.004). The linear regression has shown that eGDR decreases for 0.203 mgkg(-1)min(-1) by each increase of serum DPP4 activity of 1 U/L (p<0.001) after adjustment for adjusted for age, gender, disease duration, albuminuria and the use of antihypertensives and statins. ----- CONCLUSION: Serum DPP4 activity is associated with IR in T1DM patients and it might play an important role in its pathophysiology.

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