Methicillin-resistant S. aureus (MRSA), extended-spectrum (ESBL)- and plasmid-mediated AmpC ß-lactamase -producing Gram-negative bacteria associated with skin and soft tissue infections in hospital and community settings.

Uzunović, Selma and Bedenić, Branka and Budimir, Ana and Ibrahimagić, Amir and Kamberović, Farah and Fiolić, Zlatko and Rijnders, Michelle I. A. and Stobberingh, Ellen E. (2015) Methicillin-resistant S. aureus (MRSA), extended-spectrum (ESBL)- and plasmid-mediated AmpC ß-lactamase -producing Gram-negative bacteria associated with skin and soft tissue infections in hospital and community settings. Medicinski glasnik, 12 (2). pp. 157-68. ISSN 1840-2445

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Abstract

AIM To investigate the characteristics of meticillin-resistant S. aureus (MRSA), extended-spectrum (ESBL), and plasmid-mediated AmpC beta-lactamase producing Gram-negative bacteria causing skin and soft tissue infections (SSTIs) in hospital and outpatient settings of Zenica-Doboj Canton, Bosnia and Herzegovina. ----- METHODS Antibiotic susceptibility was determined by disc-diffusion and broth microdillution methods according to CLSI guidelines. MecA gene was detected by PCR, and genetic characterization of MRSA was performed using spa-typing and the algorithm based upon repeat patterns (BURP). Double-disk-synergy test was used to screen for ESBLs. PCR was used to detect blaESBL alleles. Genetic relatedness of the strains was tested by PFGE. ----- RESULTS Seventeen in-patients with MRSA, 13 with ESBL-producing Gram-negative bacteria and three patients co-infected with both, were detected. Five MRSA and 16 ESBL-producing Gram-negative bacteria were found in outpatient samples. Klebsiella spp. was isolated in 11 in- and seven outpatients. MLST CC152 was the most prevalent MRSA. Seven (38.9%) Klebsiella spp. yielded amplicons with primers specific for SHV, TEM-1 and CTX-M group 1 β-lactamases. Eight K. pneumonia (44.4%) and 16 (64%) MRSA (including the in- and outpatient) strains were clonally related. ----- CONCLUSION The presence of MRSA and ESBL-producing organisms causing SSTIs in the community poses a substantial concern, due to the high morbidity and mortality associated with possible consequent hospital infections.

Item Type: Article
Additional Information: Copyright © Ljekarska Komora Zeničko-Dobojskog Kantona
MeSH: Adult ; Ampicillin ; Bosnia and Herzegovina ; Child, Preschool ; Drug Resistance, Multiple, Bacterial ; Female ; Gram-Negative Bacteria/isolation & purification ; Humans ; Infant ; Inpatients ; Male ; Methicillin-Resistant Staphylococcus aureus/isolation & purification ; Middle Aged ; Skin Diseases/microbiology ; Soft Tissue Infections/microbiology ; Surgical Wound Infection/microbiology
Departments: Katedra za medicinsku mikrobiologiju i parazitologiju
Depositing User: Marijan Šember
Status: Published
Creators:
CreatorsEmail
Uzunović, SelmaUNSPECIFIED
Bedenić, BrankaUNSPECIFIED
Budimir, AnaUNSPECIFIED
Ibrahimagić, AmirUNSPECIFIED
Kamberović, FarahUNSPECIFIED
Fiolić, ZlatkoUNSPECIFIED
Rijnders, Michelle I. A.UNSPECIFIED
Stobberingh, Ellen E.UNSPECIFIED
Date: August 2015
Date Deposited: 03 Aug 2016 10:48
Last Modified: 03 Aug 2016 10:48
Subjects: /
Related URLs:
URI: http://medlib.mef.hr/id/eprint/2614

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