Repozitorij Medicinskog fakulteta Sveučilišta u Zagrebu

Correlation between Bcl-2 and Bax in atrophic and hypertrophic type of actinic keratosis

Tomas, Davor and Krušlin, Božo and Čupić, Hrvoje and Stanimirović, Andrija and Bošnjak, Berislav and Lovričević, Ivo and Belicza, Mladen (2006) Correlation between Bcl-2 and Bax in atrophic and hypertrophic type of actinic keratosis. Journal of the European Academy of Dermatology and Venereology, 20 (1). pp. 51-57. ISSN 0926-9959 (Print) 1468-3083 (Electronic)

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    English abstract

    BACKGROUND: Recent investigations consider actinic keratosis (AK) as an earliest visible pattern of squamous cell carcinoma (SCC). We have analysed the expression of apoptosis-related proteins TP53, Bcl-2 and Bax in 30 atrophic and 30 hypertrophic AK cases. ----- MATERIAL AND METHODS: Immunohistochemical analysis was performed following microwave streptavidin immunoperoxidase protocol on DAKO TechMate Horizon automated immunostainer (DAKO, Copenhagen, Denmark). Monoclonal antibody for TP53 and Bcl-2 and polyclonal antibody for Bax (DAKO, Copenhagen, Denmark) were used. ----- RESULTS: Expression of TP53 showed no significant differences between two analysed groups (chi2-test, P = 0.35636) whereas expression of Bcl-2 and Bax protein was significantly higher in atrophic compared to hypertrophic AK (chi2-test, P = 0.01458 and P = 0.00358, respectively). Comparison of Bcl-2 : Bax ratio in two analysed AK showed significantly higher value in hypertrophic compared to atrophic AK (Mann-Whitney U test, P = 0.02272). Statistical analysis did not show any correlation between patient's sex and age, localization and size of the lesion with expression of investigated oncoproteins (anova, P > 0.05). ----- CONCLUSIONS: Our results may indicate higher resistance of keratinocytes on apoptotic stimuli in hypertrophic compared to atrophic AK. Thus, we suppose that keratinocytes in hypertrophic AK live longer and probably have higher propensity for additional mutations and conversion to overt SCC.

    Item Type: Article
    MeSH: Keratosis - metabolism ; bcl-2-Associated X Protein - metabolism ; bcl-X Protein - metabolism ; Aged ; Aged, 80 and over ; Analysis of Variance ; Atrophy ; Chi-Square Distribution ; Female ; Humans ; Hypertrophy ; Immunoenzyme Techniques ; Male ; Middle Aged ; Statistics, Nonparametric ; Tumor Suppressor Protein p53 - metabolism
    Divisions: Katedra za patologiju
    Depositing User: Lea Škorić
    Status: Published
    Tomas, Davor
    Krušlin, Božo
    Čupić, Hrvoje
    Stanimirović, Andrija
    Bošnjak, Berislav
    Lovričević, Ivo
    Belicza, Mladen
    Date: January 2006
    Date Deposited: 12 Apr 2007
    Last Modified: 23 Sep 2011 18:10
    Subjects: /
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