FOXP1 expression in normal and neoplastic erythroid and myeloid cells [Ekspresija FOXP1 u normalnim i neoplastičnim stanicama ertroidne i mijeloidne loze]

Lovrić, Eva and Horvat Pavlov, Katarina and Korać, Petra and Dominis, Mara (2015) FOXP1 expression in normal and neoplastic erythroid and myeloid cells [Ekspresija FOXP1 u normalnim i neoplastičnim stanicama ertroidne i mijeloidne loze]. Collegium Antropologicum, 39 (3). pp. 755-759. ISSN 0350-6134

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Abstract

FOXP1 protein was firstly analyzed in normal tissues, and afterwards in different tumor tissues, mainly carcinoma and lymphoma. In B-cell malignancies, its role was well explored; its expression was shown to be connected with disease prognosis in certain B-non Hodgkin lymphomas. In this study, 16 bone marrow trephine samples from patients with no hematopoietic malignancies and 10 samples from peripheral blood of healthy individuals were immunostained with anti-FOXP1 antibody. Positive cells in bone marrows were not only lymphocytes, but also cells that are immunohistochemically positive for glycophorin C or myeloperoxidase. Peripheral blood samples showed no other positive cells, but small round lymphocytes. Additionally 60 samples from patients with myeloid lineage neoplasms were analyzed. 25 samples from patients with myelodysplastic syndrome (MDS) and 35 patients with myeloproliferative disease (MPD) were double immunostained with anti-FOXP1/anti-glycophorin C and anti-FOXP1/anti-myeloperoxidase antibodies. FOXP1 was found to be expressed in 22 cases of MDS and in none of MPD cases. Its expression in MDS was observed mostly in myeloperoxidase positive cells in contrast to gylcophorin C positive cells. Only two cases revealed both myeloperoxidase positive cells and gylcophorin C positive cells expressing FOXP1 transcription factor. Our results show that FOXP1 is present in normal cells of erythroid and myeloid linages and thus suggest its possible role in development of all hematopoetic cells as well as possible involvement in neoplasm development of myeloid disorders.

Abstract in Croatian

Protein FOXP1 prvo je bio istraživan u normalnom tkivu i različitim tumorskim tkivima, najviše karcinomima i limfomima. Njegova je uloga u B-staničnim neoplazmama dobro istražena, a dokazano je i da je njegova ekspresija u pojedinim B ne-Hodgkinovim limfomima vezana za prognozu. U ovom istraživanju 16 uzoraka biopsija koštanih srži uklopljenih u parafin pacijanata bez hematoloških bolesti i 10 uzoraka razmaza periferne krvi zdravih ispitanika, imunohistokemijski su obojeni anti-FOXP1 antitijelom. Pozitivne stanice u koštanim sržima nisu bili samo limfociti, već i stanice koje su imunohistokemijski pozitivne i na glikoforin C ili mijeloperoksidazu. Uzorci razmaza periferne krvi nisu pokazivali druge pozitivne stanice osim malih, okruglih limfocita. Dodatno je analizirano 60 uzoraka koštanih srži pacijenata s neoplazmama mijeloidne linije. 25 uzoraka bilo je od pacijenata s mijelodisplastičnim sindromom (MDS), a 35 s mijeloproliferativnom bolesti (MPD). Svi su uzorci obojeni dvostrukim imunohistokemijskim bojenjem korištenjem anti-FOXP1/anti-glikoforin C i anti-FOXP1/anti-mijeloperoksidaza antitijela. FOXP1 nađen je u neoplastičnim stanicama 22 uzorka MDS-a, dok tumorske stanice MPD-a nisu pokazivale njegovu ekspresiju. Neoplastične MDS stanice koje eksprimiraju FOXP1 većinom su bile pozitivne i na mijeoperoksidazu. Samo su dva uzorka pokazala ekspresiju FOXP1 proteina i u stanicama pozitivnim na glikoforin C i u stanicama pozitivnim na mijeloperoksidazu. Ovakvi rezultati pokazuju prisutnost FOXP1 proteina u normalnim razvojnim stadijima eritroidne i mijeloidne loze i ukazuju na mogućnost da je on potreban za razvoj svih hematopoetskih stanica. Isto tako ukazuju na mogućnost da FOXP1 ima ulogu u razvoju mijeloidnih neoplazmi.

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